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Literature summary extracted from

  • Kleczkowski, L.A.
    Kinetics and regulation of the NAD(P)H-dependent glyoxylate-specific reductase from spinach leaves (1995), Z. Naturforsch. C, 50, 21-28.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.79 phosphate 20 mM, 17% activation Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 2-mercaptoethanol 5 mM, 37% inhibition, incubation for 1 min Spinacia oleracea
1.1.1.79 alpha-ketoglutarate 5 mM, 57% inhibition Spinacia oleracea
1.1.1.79 ATP 5 mM, 28% inhibition Spinacia oleracea
1.1.1.79 Carbonate 20 mM, 7-16% inhibition Spinacia oleracea
1.1.1.79 chloride 20 mM, 7-16% inhibition Spinacia oleracea
1.1.1.79 cyanide 2 mM, 46% inhibition, 20 mM, 92% inhibition Spinacia oleracea
1.1.1.79 diethyldicarbonate 1 mM, 33% inhibition, incubation for 1 min Spinacia oleracea
1.1.1.79 dithiothreitol 5 mM, 51% inhibition, incubation for 1 min Spinacia oleracea
1.1.1.79 glycolate 5 mM, 42% inhibition Spinacia oleracea
1.1.1.79 iodoacetamide 1 mM, 28% inhibition, incubation for 1 min Spinacia oleracea
1.1.1.79 NADP+ competitive product inhibition Spinacia oleracea
1.1.1.79 nitrate 20 mM, 7-16% inhibition Spinacia oleracea
1.1.1.79 nitrite 20 mM, 7-16% inhibition Spinacia oleracea
1.1.1.79 PMSF 1 mM, 30% inhibition, incubation for 1 min Spinacia oleracea
1.1.1.79 pyruvate 5 mM, 33% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.006
-
 NADPH
-
 Spinacia oleracea
1.1.1.79 0.059
-
 glyoxylate cofactor NADPH Spinacia oleracea
1.1.1.79 0.3
-
 NADH
-
 Spinacia oleracea
1.1.1.79 1.1
-
 glyoxylate cofactor NADH Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.79 cytosol
-
 Spinacia oleracea 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H Spinacia oleracea
-
 glycolate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.79 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H
-
 Spinacia oleracea glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NADH + H+
-
 Spinacia oleracea glycolate + NAD+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NAD(P)H highly specific for NADPH Spinacia oleracea
1.1.1.79 NADPH highly specific for NADPH Spinacia oleracea