Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Conlin, C.A.; Miller, C.G.
    Dipeptidyl carboxypeptidase and oligopeptidase A from Escherichia coli and Salmonella typhimurium (1995), Methods Enzymol., 248, 567-579.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.15.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.4.15.5
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.5 captopril
-
 Escherichia coli
3.4.15.5 captopril
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.4.15.5 EDTA
-
 Escherichia coli
3.4.15.5 EDTA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 Cu2+ 0.1 mM Escherichia coli
3.4.24.70 Cu2+ 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 EDTA 1 mM Escherichia coli
3.4.24.70 EDTA 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 Ni2+ 1 mM Escherichia coli
3.4.24.70 Ni2+ 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 Zn2+ 0.1 mM Escherichia coli
3.4.24.70 Zn2+ 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.70 cytoplasm
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5737
-
3.4.24.70 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.15.5 Co2+ activates Salmonella enterica subsp. enterica serovar Typhimurium
3.4.15.5 Co2+ activates Escherichia coli
3.4.15.5 Zinc metallopeptidase Salmonella enterica subsp. enterica serovar Typhimurium
3.4.15.5 Zinc metallopeptidase Escherichia coli
3.4.24.70 Co2+ activation, 0.01 mM Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 Co2+ activation, 0.01 mM Escherichia coli
3.4.24.70 Mn2+ activation, to a lesser extent than Co2+, 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 Mn2+ activation, to a lesser extent than Co2+, 0.1 mM Escherichia coli
3.4.24.70 additional information putative Zn2+-binding site Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 additional information putative Zn2+-binding site Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.5 77500
-
 E. coli, calculation from nucleotide sequence Escherichia coli
3.4.15.5 78000
-
 1 * 78000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.15.5 Acetyl-Ala3 + H2O Salmonella enterica subsp. enterica serovar Typhimurium the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source ?
-
 ?
3.4.15.5 Acetyl-Ala3 + H2O Escherichia coli the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source ?
-
 ?
3.4.15.5 Acetyl-Ala3 + H2O Escherichia coli TN3080 the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source ?
-
 ?
3.4.15.5 additional information Salmonella enterica subsp. enterica serovar Typhimurium may have an important function in degradation of intracellular proteins ?
-
 ?
3.4.15.5 additional information Escherichia coli may have an important function in degradation of intracellular proteins ?
-
 ?
3.4.15.5 additional information Escherichia coli TN3080 may have an important function in degradation of intracellular proteins ?
-
 ?
3.4.24.70 Bacteriophage P22 gp7 protein + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
3.4.24.70 Prolipoprotein signal peptide + H2O Salmonella enterica subsp. enterica serovar Typhimurium in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides ?
-
 ?
3.4.24.70 Prolipoprotein signal peptide + H2O Escherichia coli in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.5 Escherichia coli
-
-
-
3.4.15.5 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
3.4.24.70 Escherichia coli
-
 TN3080
-
3.4.24.70 Escherichia coli TN3080
-
 TN3080
-
3.4.24.70 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.4.15.5
-
 Escherichia coli
3.4.24.70
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.5 Acetyl-Ala-Ala-Ala + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Acetyl-Ala + Ala-Ala
-
 ?
3.4.15.5 Acetyl-Ala-Ala-Ala + H2O
-
 Escherichia coli Acetyl-Ala + Ala-Ala
-
 ?
3.4.15.5 Acetyl-Ala-Ala-Ala + H2O
-
 Escherichia coli TN3080 Acetyl-Ala + Ala-Ala
-
 ?
3.4.15.5 Acetyl-Ala3 + H2O the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.15.5 Acetyl-Ala3 + H2O the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source Escherichia coli ?
-
 ?
3.4.15.5 Acetyl-Ala3 + H2O the enzyme is required for the utilization of acetyl-Ala3 as a sole nitrogen source Escherichia coli TN3080 ?
-
 ?
3.4.15.5 additional information enzyme requires a free C terminus, and it cannot hydrolyze bonds in which the peptide nitrogen is donated by proline or in which both amino acids are Gly Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.15.5 additional information enzyme requires a free C terminus, and it cannot hydrolyze bonds in which the peptide nitrogen is donated by proline or in which both amino acids are Gly Escherichia coli ?
-
 ?
3.4.15.5 additional information may have an important function in degradation of intracellular proteins Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.15.5 additional information may have an important function in degradation of intracellular proteins Escherichia coli ?
-
 ?
3.4.15.5 additional information enzyme requires a free C terminus, and it cannot hydrolyze bonds in which the peptide nitrogen is donated by proline or in which both amino acids are Gly Escherichia coli TN3080 ?
-
 ?
3.4.15.5 additional information may have an important function in degradation of intracellular proteins Escherichia coli TN3080 ?
-
 ?
3.4.24.70 Acetyl-Ala-Ala-Ala-Ala + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Ala-Ala-Ala + acetyl-Ala
-
 ?
3.4.24.70 Acetyl-Ala-Ala-Ala-Ala + H2O
-
 Escherichia coli Ala-Ala-Ala + acetyl-Ala
-
 ?
3.4.24.70 Bacteriophage P22 gp7 protein + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.24.70 Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
-
 ?
3.4.24.70 Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
-
 Escherichia coli Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
-
 ?
3.4.24.70 Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Benzyloxycarbonyl-Gly-Pro-Gly + Gly-Pro-Ala
-
 ?
3.4.24.70 Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Escherichia coli Benzyloxycarbonyl-Gly-Pro-Gly + Gly-Pro-Ala
-
 ?
3.4.24.70 additional information benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.24.70 additional information benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly Escherichia coli ?
-
 ?
3.4.24.70 additional information benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly Escherichia coli TN3080 ?
-
 ?
3.4.24.70 Prolipoprotein signal peptide + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Hydrolyzed prolipoprotein signal peptide smallest fragment: tripeptide ?
3.4.24.70 Prolipoprotein signal peptide + H2O
-
 Escherichia coli Hydrolyzed prolipoprotein signal peptide smallest fragment: tripeptide ?
3.4.24.70 Prolipoprotein signal peptide + H2O in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.4.24.70 Prolipoprotein signal peptide + H2O in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.15.5 monomer 1 * 78000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.70 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
3.4.24.70 37
-
 assay at Escherichia coli