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Literature summary extracted from

  • Tamaoki, B-i.; Inano, H.
    Testicular 17beta-hydroxysteroid dehydrogenase: its distribution, purification and properties (1975), J. Steroid Biochem., 6, 361-363.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.64 0.0083
-
 NADPH
-
 Sus scrofa
1.1.1.64 0.04
-
 androstenedione
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.64 35500
-
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.64 17beta-estradiol + NADP+ Rattus norvegicus
-
 estrone + NADPH
-
 r
1.1.1.64 17beta-estradiol + NADP+ Sus scrofa
-
 estrone + NADPH
-
 r
1.1.1.64 testosterone + NADP+ Rattus norvegicus
-
 androst-4-ene-3,17-dione + NADPH + H+
-
 r
1.1.1.64 testosterone + NADP+ Sus scrofa
-
 androst-4-ene-3,17-dione + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.64 Rattus norvegicus
-
-
-
1.1.1.64 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.64 ammonium sulfate, Sephadex G-100, DEAE-cellulose, Bio-Gel P-100 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.64 testis
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.64 0.0033
-
 enzyme activity in smooth-surfaced microsomes Rattus norvegicus
1.1.1.64 67.7
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.64 -20°C, 6 months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.64 17beta-estradiol + NADP+
-
 Rattus norvegicus estrone + NADPH
-
 r
1.1.1.64 17beta-estradiol + NADP+
-
 Sus scrofa estrone + NADPH
-
 r
1.1.1.64 testosterone + NADP+
-
 Rattus norvegicus androst-4-ene-3,17-dione + NADPH + H+
-
 r
1.1.1.64 testosterone + NADP+
-
 Sus scrofa androst-4-ene-3,17-dione + NADPH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.64 50
-
 in phosphate buffer at pH 7.4 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.64 6.5 7.5 at 37°C Sus scrofa
1.1.1.64 8.5
-
 at 50°C Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.64 NADP+
-
 Rattus norvegicus
1.1.1.64 NADP+
-
 Sus scrofa