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Literature summary extracted from

  • Markert, C.; Träger, L.
    Enzyminduktion bei Streptomyces hydrogenans, V. Charakterisierung der Testosteron-17beta-Dehydrogenase und ihre Induktion durch Steroide (1975), Hoppe-Seyler's Z. Physiol. Chem., 356, 1843-1852.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.239 17alpha-methyltestosterone competitive inhibition Streptomyces exfoliatus
1.1.1.239 androstendione
-
 Streptomyces exfoliatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.239 0.013
-
 testosterone
-
 Streptomyces exfoliatus
1.1.1.239 0.021
-
 17beta-estradiol
-
 Streptomyces exfoliatus
1.1.1.239 0.125
-
 NAD+
-
 Streptomyces exfoliatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.239 130000
-
 partly purified enzyme, gel filtration Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.239 testosterone + NAD+ Streptomyces exfoliatus
-
 androst-4-ene-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.239 Streptomyces exfoliatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.239 Sephadex G-200, DEAE-cellulose, 9fold purification Streptomyces exfoliatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.239 17beta-hydroxy-1alpha-methyl-5alpha-androstan-3-one + NAD+ 192% velocity compared to testosterone Streptomyces exfoliatus 1alpha-methyl-5alpha-androstane-3,17-dione + NADH
-
 r
1.1.1.239 3beta-hydroxy-5alpha-androstane-17-one + NAD+ 75% velocity compared to testosterone Streptomyces exfoliatus 5alpha-androstane-3,17-dione + NADH
-
 r
1.1.1.239 5alpha-androstane-3alpha,17beta-diol + NAD+ 204% velocity compared to testosterone Streptomyces exfoliatus 5alpha-androstane-3alpha-ol-17-one + NADH
-
 r
1.1.1.239 5alpha-androstane-3beta,17beta-diol + NAD+ 17% velocity compared to testosterone Streptomyces exfoliatus 5alpha-androstane-3beta-ol-17-one + NADH
-
 r
1.1.1.239 5alpha-dihydrotestosterone + NAD+ 119% initial velocity compared to testosterone Streptomyces exfoliatus androstanedione + NADH + H+
-
 r
1.1.1.239 5beta-dihydrotestosterone + NAD+ 50% initial velocity compared to testosterone Streptomyces exfoliatus androstanedione + NADH + H+
-
 r
1.1.1.239 estradiol + NAD+ 15% velocity compared to testosterone oxidation Streptomyces exfoliatus estrone + NADH
-
 r
1.1.1.239 testosterone + NAD+
-
 Streptomyces exfoliatus androst-4-ene-3,17-dione + NADH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.239 45
-
-
 Streptomyces exfoliatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.239 7.4 9.5
-
 Streptomyces exfoliatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.239 NAD+
-
 Streptomyces exfoliatus