Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gotto, A.M.; Kornberg, H.L.
    The metabolism of C2 compounds in micro-organisms (1961), Biochem. J., 81, 273-284.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.60
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.60 fluoroacetate
-
 Pseudomonas putida
1.1.1.60 glycolate
-
 Pseudomonas putida
1.1.1.60 glyoxylate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.60 0.02 0.027 NADH
-
 Pseudomonas putida
1.1.1.60 0.036 0.05 NADPH
-
 Pseudomonas putida
1.1.1.60 0.05 0.4 D-glycerate
-
 Pseudomonas putida
1.1.1.60 0.05 0.4 tartronate semialdehyde
-
 Pseudomonas putida
1.1.1.60 0.2
-
 malonic semialdehyde
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.60 91000
-
 sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.60 tartronate semialdehyde + NAD(P)H Pseudomonas putida
-
 (R)-glycerate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.60 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.60
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.60 160
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.60 2°C, suspension in ammonium sulfate, pH 7.5, 10% loss in 1 month Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.60 hydroxypyruvate + NAD(P)H + H+
-
 Pseudomonas putida glycerate + NAD(P)+
-
 r
1.1.1.60 malonic semialdehyde + NADH
-
 Pseudomonas putida 3-hydroxypropanoate + NAD+
-
 ?
1.1.1.60 mesoxalic semialdehyde + NADH
-
 Pseudomonas putida hydroxypyruvate + NAD+
-
 ?
1.1.1.60 tartronate semialdehyde + NAD(P)H
-
 Pseudomonas putida (R)-glycerate + NAD(P)+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.60 243
-
 NADH
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.60 6.2 8.7
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.60 NAD+
-
 Pseudomonas putida
1.1.1.60 NADH
-
 Pseudomonas putida
1.1.1.60 NADP+
-
 Pseudomonas putida
1.1.1.60 NADPH
-
 Pseudomonas putida