EC Number | General Stability | Organism |
---|---|---|
1.1.1.56 | NAD+ improves stability and reverses inhibition | Klebsiella aerogenes |
1.1.1.56 | unstable in dilute solutions | Klebsiella aerogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.56 | 2-mercaptoethanol | competitive to ribitol | Klebsiella aerogenes | |
1.1.1.56 | diethyldithiocarbamate | 50% inhibition at a concentration of 50 mM | Klebsiella aerogenes | |
1.1.1.56 | dithiothreitol | - |
Klebsiella aerogenes | |
1.1.1.56 | Hg2+ | protection by NAD+ | Klebsiella aerogenes | |
1.1.1.56 | o-phenanthroline | 50% inhibition at a concentration of 50 mM | Klebsiella aerogenes | |
1.1.1.56 | p-chloromercuribenzoate | - |
Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.56 | 0.1 | - |
NAD+ | pH 11 | Klebsiella aerogenes | |
1.1.1.56 | 5 | - |
ribitol | pH 11 | Klebsiella aerogenes | |
1.1.1.56 | 1000 | - |
xylitol | pH 11 | Klebsiella aerogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.56 | additional information | no requirement for metal ions | Klebsiella aerogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.56 | 27000 | - |
4 * 27000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
1.1.1.56 | 100000 | 110000 | wild type, gel filtration | Klebsiella aerogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.56 | Klebsiella aerogenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.56 | wild type | Klebsiella aerogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.56 | 83 | - |
wild type enzyme | Klebsiella aerogenes |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.56 | -20°C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0 | Klebsiella aerogenes |
1.1.1.56 | 2°C presence of NAD+ several days | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.56 | ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH | - |
? | |
1.1.1.56 | xylitol + NAD+ | - |
Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.56 | tetramer | 4 * 27000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.56 | 28 | - |
- |
Klebsiella aerogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.56 | 11 | - |
alcohol oxidation | Klebsiella aerogenes |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.56 | 10 | 11 | unstable at high pH values | Klebsiella aerogenes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.56 | NAD+ | - |
Klebsiella aerogenes | |
1.1.1.56 | NADH | - |
Klebsiella aerogenes |