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Literature summary extracted from

  • Taylor, S.S.; Rigby, P.W.J.; Hartley, B.S.
    Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure (1974), Biochem. J., 141, 693-700.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.56 NAD+ improves stability and reverses inhibition Klebsiella aerogenes
1.1.1.56 unstable in dilute solutions Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.56 2-mercaptoethanol competitive to ribitol Klebsiella aerogenes
1.1.1.56 diethyldithiocarbamate 50% inhibition at a concentration of 50 mM Klebsiella aerogenes
1.1.1.56 dithiothreitol
-
Klebsiella aerogenes
1.1.1.56 Hg2+ protection by NAD+ Klebsiella aerogenes
1.1.1.56 o-phenanthroline 50% inhibition at a concentration of 50 mM Klebsiella aerogenes
1.1.1.56 p-chloromercuribenzoate
-
Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.56 0.1
-
NAD+ pH 11 Klebsiella aerogenes
1.1.1.56 5
-
ribitol pH 11 Klebsiella aerogenes
1.1.1.56 1000
-
xylitol pH 11 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.56 additional information no requirement for metal ions Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.56 27000
-
4 * 27000, wild type enzyme, SDS-PAGE Klebsiella aerogenes
1.1.1.56 100000 110000 wild type, gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.56 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.56 wild type Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.56 83
-
wild type enzyme Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.56 -20°C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0 Klebsiella aerogenes
1.1.1.56 2°C presence of NAD+ several days Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.56 ribitol + NAD+
-
Klebsiella aerogenes D-ribulose + NADH
-
?
1.1.1.56 xylitol + NAD+
-
Klebsiella aerogenes D-xylulose + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.56 tetramer 4 * 27000, wild type enzyme, SDS-PAGE Klebsiella aerogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.56 28
-
-
Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.56 11
-
alcohol oxidation Klebsiella aerogenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.56 10 11 unstable at high pH values Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.56 NAD+
-
Klebsiella aerogenes
1.1.1.56 NADH
-
Klebsiella aerogenes