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Literature summary extracted from

  • Guan, G.; Todo, T.; Tanaka, M.; Young, G.; Nagahama, Y.
    Isoleucine-15 of rainbow trout carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase is critical for coenzyme (NADPH) binding (2000), Proc. Natl. Acad. Sci. USA, 97, 3079-3083.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.53 expression in Escherichia coli Oncorhynchus mykiss

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.53 I15T in 20beta-HSD type A, cofactor binding is abolished Oncorhynchus mykiss
1.1.1.53 Q27K in 20beta-HSD type B, cofactor binding is reduced Oncorhynchus mykiss
1.1.1.53 T15I in 20beta-HSD type B, enzyme activity is restored Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ Oncorhynchus mykiss
-
 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Oncorhynchus mykiss
-
 2 types of enzyme activity: carbonyl reductase + 20beta-hydroxysteroid dehydrogenase (type A) or only 20beta-hydroxysteroid dehydrogenase activity (type B)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.53 recombinant from Escherichia coli Oncorhynchus mykiss

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.53 androstan-3alpha,17beta-diol + NAD+ = 17beta-hydroxyandrostan-3-one + NADH + H+ exhibits carbonyl reductase-like activity Oncorhynchus mykiss
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.53 ovarian follicle ovarian Oncorhynchus mykiss
-
1.1.1.53 ovary
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.53 0.27
-
 enzyme type B mutant Q27K, 17alpha-hydroxyprogesterone Oncorhynchus mykiss
1.1.1.53 1.57
-
 enzyme type A, 17alpha-hydroxyprogesterone Oncorhynchus mykiss
1.1.1.53 1.64
-
 enzyme type B mutant T15I, 17alpha-hydroxyprogesterone Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ enzyme wild type A and B, mutants of enzyme type A and B Oncorhynchus mykiss 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
 ?
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Oncorhynchus mykiss 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?