Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ohno, S.; Nakajin, S.; Shinoda, M.
    20beta-Hydroxysteroid dehydrogenase of neonatal pig testis: 3alpha/beta-hydroxysteroid dehydrogenase activities catalyzed by highly purified enzyme (1991), J. Steroid Biochem. Mol. Biol., 38, 787-794.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.53 Cd2+
-
 Sus scrofa
1.1.1.53 Cu2+
-
 Sus scrofa
1.1.1.53 Fe2+
-
 Sus scrofa
1.1.1.53 Hg2+
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.53 0.00015
-
 progesterone reduction Sus scrofa
1.1.1.53 0.00016
-
 5alpha-pregnane-3,20-dione reduction Sus scrofa
1.1.1.53 0.00018
-
 5alpha-androstan-17beta-ol-3-one reduction Sus scrofa
1.1.1.53 0.00032
-
 5alpha-Androstan-3alpha-ol-17-one oxidation Sus scrofa
1.1.1.53 0.00094
-
 17alpha-hydroxyprogesterone reduction Sus scrofa
1.1.1.53 7.2
-
 beta-NADPH
-
 Sus scrofa
1.1.1.53 14.8
-
 beta-NADP+
-
 Sus scrofa
1.1.1.53 88.2
-
 beta-NAD+
-
 Sus scrofa
1.1.1.53 126.1
-
 alpha-NADH
-
 Sus scrofa
1.1.1.53 294.8
-
 beta-NADH
-
 Sus scrofa
1.1.1.53 328
-
 alpha-NADPH
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.53
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.53 testis
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.53 0.003
-
 purified enzyme, NADPH, progesterone Sus scrofa
1.1.1.53 0.019
-
 purified enzyme, NADPH, 5beta-androstan-3alpha,17beta-diol oxidation Sus scrofa
1.1.1.53 0.023
-
 purified enzyme, NADPH, dehydroisoandrosterone Sus scrofa
1.1.1.53 0.025
-
 purified enzyme, NADPH, 5alpha-androstan-3alpha-ol-17-one oxidation Sus scrofa
1.1.1.53 0.046
-
 purified enzyme, NADPH, 5alpha-androstan-17beta-ol-3-one Sus scrofa
1.1.1.53 0.051
-
 purified enzyme, NADPH, 5alpha-pregnane-3,20-dione Sus scrofa
1.1.1.53 0.052
-
 purified enzyme, NADPH, 5alpha-androstan-3alpha,17beta-diol oxidation Sus scrofa
1.1.1.53 0.091
-
 purified enzyme, NADPH, 17alpha-methyl-5alpha-androstan-17beta-ol-3-one Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Sus scrofa 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
 ?
1.1.1.53 5alpha-androstan-17beta-ol-3-one + NAD(P)H + H+ i.e. 5alpha-dihydrotestosterone Sus scrofa 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ ratio 4:3 ?
1.1.1.53 5alpha-androstan-3alpha-ol-17-one + alpha-NAD+
-
 Sus scrofa 5alpha-dihydrotestosterone + alpha-NADH + H+
-
 r
1.1.1.53 5alpha-androstan-3alpha-ol-17-one + beta-NAD+
-
 Sus scrofa 5alpha-dihydrotestosterone + beta-NADH + H+
-
 r
1.1.1.53 5alpha-androstane-3,17-dione + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.53 5alpha-dihydrotestosterone + alpha-NADH + H+
-
 Sus scrofa 5alpha-androstan-3alpha-ol-17-one + alpha-NAD+
-
 r
1.1.1.53 5alpha-dihydrotestosterone + alpha-NADPH + H+
-
 Sus scrofa 5alpha-androstan-3alpha-ol-17-one + alpha-NADP+
-
 r
1.1.1.53 5alpha-dihydrotestosterone + beta-NADH + H+
-
 Sus scrofa 5alpha-androstan-3alpha-ol-17-one + beta-NAD+
-
 r
1.1.1.53 5alpha-pregnane-3,20-dione + NADPH
-
 Sus scrofa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.53 5
-
 reduction of 5alpha-androstan-17beta-ol-3-one with beta-NADPH Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.53 4.5 9
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.53 NAD+
-
 Sus scrofa
1.1.1.53 NADH
-
 Sus scrofa
1.1.1.53 NADP+
-
 Sus scrofa
1.1.1.53 NADPH hydrogen transfer is stereospecific Sus scrofa