Any feedback?
Literature summary extracted from
- Bifunctional enzyme activity at the same active site: study of 3alpha and 20beta activity by affinity alkylation of 3alpha, 20beta-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids (1980), Biochemistry, 19, 978-986.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.53 | 17-Acetoxyprogesterone | - |
Streptomyces exfoliatus |  |
| 1.1.1.53 | 17-Bromoacetoxyprogesterone | - |
Streptomyces exfoliatus | |
| 1.1.1.53 | 5alpha-Dihydrotestosterone bromoacetate | - |
Streptomyces exfoliatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.53 | 0.0039 | - |
progesterone | - |
Streptomyces exfoliatus | |
| 1.1.1.53 | 0.0045 | - |
5alpha-pregnane-3,20-dione | - |
Streptomyces exfoliatus | |
| 1.1.1.53 | 0.0105 | - |
17-hydroxyprogesterone | - |
Streptomyces exfoliatus | |
| 1.1.1.53 | 0.02 | - |
6beta-[bromoacetoxy]-progesterone | - |
Streptomyces exfoliatus | |
| 1.1.1.53 | 0.318 | - |
cortisone | - |
Streptomyces exfoliatus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.53 | Streptomyces exfoliatus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.53 | commercial preparation | - |
Streptomyces exfoliatus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.53 | 17-hydroxyprogesterone + NADH | - |
Streptomyces exfoliatus | ? | - |
? | |
| 1.1.1.53 | 17alpha-hydroxyprogesterone + NADPH + H+ | - |
Streptomyces exfoliatus | 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ | - |
? | |
| 1.1.1.53 | 5alpha-pregnane-3,20-dione + NADH | - |
Streptomyces exfoliatus | ? | - |
? | |
| 1.1.1.53 | 6beta-(bromoacetoxy)-progesterone + NADH | - |
Streptomyces exfoliatus | ? | - |
? | |
| 1.1.1.53 | 6beta-[bromoacetoxy]-progesterone + NAD+ | - |
Streptomyces exfoliatus | ? | - |
r | |
| 1.1.1.53 | cortisone + NAD(P)H | i.e. 17beta,21-dihydroxy-pregn-4-ene-3,11,20-trione | Streptomyces exfoliatus | 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+ | - |
? | |
| 1.1.1.53 | progesterone + NAD(P)H | - |
Streptomyces exfoliatus | 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ | - |
? | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.53 | 9 | - |
in Tris-buffer, best value for stability | Streptomyces exfoliatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
