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Literature summary extracted from

  • Nakajin, S.; Ohno, S.; Shinoda, M.
    20beta-Hydroxysteroid dehydrogenase of neonatal pig testis: purification and some properties (1988), J. Biochem., 104, 565-569.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.53 Cu2+
-
 Sus scrofa
1.1.1.53 cysteine slight Sus scrofa
1.1.1.53 dithiothreitol slight Sus scrofa
1.1.1.53 Hg2+
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.53 0.0015
-
 progesterone
-
 Sus scrofa
1.1.1.53 0.004
-
 pregnenolone
-
 Sus scrofa
1.1.1.53 0.0086
-
 deoxycorticosterone
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.53 30500
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ Sus scrofa
-
 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+
-
 ?
1.1.1.53 progesterone + NADPH Sus scrofa
-
 20beta-hydroxy-pregn-4-ene-3-one + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.53
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.53 testis
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.53 0.004
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.53 -20°C, 50 mM potassium phosphate buffer, pH 7.4 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 17alpha-hydroxypregnenolone + NADPH i.e. 3beta,17alpha-dihydroxypregn-5-ene-20-one Sus scrofa 3beta,17alpha,20beta-trihydroxypregn-5-ene + NADP+
-
 ?
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Sus scrofa 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
 ?
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Sus scrofa 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+
-
 ?
1.1.1.53 deoxycorticosterone + NADPH i.e. 21-hydroxypregn-4-ene-3,20-dione, 21-hydroxyprogesterone Sus scrofa 20,21-dihydroxypregn-4-ene-3-one + NADP+
-
 ?
1.1.1.53 deoxycortisol + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.53 pregnenolone + NADPH i.e. 3-hydroxypregn-5-en-20-one Sus scrofa 3beta,20beta-Dihydroxypregn-5-ene + NADP+
-
 ?
1.1.1.53 progesterone + NAD(P)H
-
 Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+
-
 ?
1.1.1.53 progesterone + NADPH
-
 Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.53 monomer 1 * 30500, SDS-PAGE with and without 2-mercaptoethanol Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.53 50
-
-
 Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.53 35 60 50% of maximal activity at 35°C, 10.4% of maximal activity at 60°C Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.53 45
-
 up to Sus scrofa
1.1.1.53 55
-
 inactivation at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.53 5.5
-
 reduction of oxosteroid Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.53 4.5 5.5
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.53 6 10
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.53 NADPH
-
 Sus scrofa