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Literature summary extracted from

  • Stokes, N.A.; Hylemon, P.B.
    Characterization of DELTA4-3-ketosteroid-5beta-reductase and 3beta-hydroxysteroid dehydrogenase in cell extracts of Clostridium innocuum (1985), Biochim. Biophys. Acta, 836, 255-261.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.99.6 FMNH2 slightly stimulatory in combination with NADH [Clostridium] innocuum
1.3.99.6 FMNH2 slightly stimulatory in combination with NADH Clostridium paraputrificum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.51 0.21
-
 5beta-pregnan-3,20-dione
-
 [Clostridium] innocuum
1.3.99.6 0.042
-
 4-pregnen-3,20-dione
-
 [Clostridium] innocuum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.51 cytosol
-
 [Clostridium] innocuum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.51 80000
-
 gel filtration [Clostridium] innocuum
1.3.99.6 80000
-
 gel filtration [Clostridium] innocuum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 [Clostridium] innocuum
-
-
-
1.3.99.6 Clostridium paraputrificum
-
-
-
1.3.99.6 [Clostridium] innocuum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.99.6 oxygen-sensitive [Clostridium] innocuum
1.3.99.6 oxygen-sensitive Clostridium paraputrificum

Storage Stability

EC Number Storage Stability Organism
1.1.1.51 -70°C, very labile in all extracts, inactivation in 5 days, but only 30% loss of activity if 20% glycerol is included [Clostridium] innocuum
1.3.99.6 -70°C, 20% glycerol, 1 week, less than 30% loss of activity [Clostridium] innocuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 5beta-pregnan-3,20-dione + NADH
-
 [Clostridium] innocuum 3beta-hydroxy-5beta-pregnan-20-one + NAD+
-
 ?
1.3.99.6 4-pregnen-3,20-dione + NADH
-
 Clostridium paraputrificum 5beta-pregnan-3,20-dione + NAD+
-
 ?
1.3.99.6 4-pregnen-3,20-dione + NADH i.e. progesterone [Clostridium] innocuum 5beta-pregnan-3,20-dione + NAD+
-
 ?
1.3.99.6 testosterone + NADH
-
 [Clostridium] innocuum 17beta-hydroxy-5beta-androstan-3-one + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.51 6 7.5
-
 [Clostridium] innocuum
1.3.99.6 5
-
-
 [Clostridium] innocuum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 additional information
-
 [Clostridium] innocuum
1.1.1.51 NADH
-
 [Clostridium] innocuum
1.3.99.6 NADH required [Clostridium] innocuum
1.3.99.6 NADH required Clostridium paraputrificum