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Literature summary extracted from

  • Wong, T.Y.; Yao, X.T.
    The deLey-Doudoroff pathway of galactose metabolism in Azotobacter vinelandii (1994), Appl. Environ. Microbiol., 60, 2065-2068.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.48 0.125
-
 NAD+
-
 Azotobacter vinelandii
1.1.1.48 0.14
-
 NAD+
-
 Pelomonas saccharophila
1.1.1.48 0.24
-
 NAD+
-
 Pseudomonas fluorescens
1.1.1.48 0.5
-
 D-galactose
-
 Azotobacter vinelandii
1.1.1.48 0.7
-
 D-galactose
-
 Pseudomonas fluorescens
1.1.1.48 1
-
 D-galactose
-
 Pelomonas saccharophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.48 cytoplasm
-
 Pseudomonas fluorescens 5737
-
1.1.1.48 cytoplasm
-
 Azotobacter vinelandii 5737
-
1.1.1.48 cytoplasm
-
 Pelomonas saccharophila 5737
-
1.1.1.48 cytoplasm
-
 Rhizobium meliloti L5-30 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.48 74000
-
 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens
-
 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+ Azotobacter vinelandii
-
 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+ Pelomonas saccharophila
-
 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+ Rhizobium meliloti L5-30
-
 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NADP+ Pseudomonas fluorescens
-
 D-galactono-1,5-lactone + NADPH
-
 ?
1.1.1.48 D-galactose + NADP+ Rhizobium meliloti L5-30
-
 D-galactono-1,5-lactone + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Azotobacter vinelandii
-
-
-
1.1.1.48 Pelomonas saccharophila
-
-
-
1.1.1.48 Pseudomonas fluorescens
-
-
-
1.1.1.48 Rhizobium meliloti L5-30
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.48 Mono Q column, partially purified enzyme Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.48 36
-
-
 Rhizobium meliloti L5-30
1.1.1.48 76
-
 substrate L-arabinose Rhizobium meliloti L5-30
1.1.1.48 202
-
-
 Pelomonas saccharophila
1.1.1.48 260
-
-
 Azotobacter vinelandii
1.1.1.48 272
-
 coenzyme NADP+ Rhizobium meliloti L5-30
1.1.1.48 390
-
 substrate L-arabinose Azotobacter vinelandii
1.1.1.48 849
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 6-deoxy-alpha-D-galactose + NAD+
-
 Pseudomonas fluorescens 6-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 6-deoxy-alpha-D-galactose + NAD+
-
 Azotobacter vinelandii 6-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 6-deoxy-alpha-D-galactose + NAD+
-
 Pelomonas saccharophila 6-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Azotobacter vinelandii D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Pelomonas saccharophila D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Rhizobium meliloti L5-30 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NADP+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NADPH
-
 ?
1.1.1.48 D-galactose + NADP+
-
 Rhizobium meliloti L5-30 D-galactono-1,5-lactone + NADPH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Pseudomonas fluorescens L-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Pelomonas saccharophila L-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Rhizobium meliloti L5-30 L-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+ 1 mM, better substrate than D-galactose Azotobacter vinelandii L-arabinono-1,4-lactone + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.48 9
-
-
 Azotobacter vinelandii
1.1.1.48 9.1 9.5
-
 Pseudomonas fluorescens