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Literature summary extracted from

  • Bridges, R.B.; Palumbo, M.P.; Wittenberger, C.L.
    Purification and properties of an NADP-specific 6-phosphogluconate dehydrogenase from Streptococcus faecalis (1975), J. Biol. Chem., 250, 6093-6100.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Cyberlindnera jadinii
1.1.1.44 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Enterococcus faecalis
1.1.1.44 D-fructose 1,6-diphosphate
-
 Enterococcus faecalis
1.1.1.44 D-fructose 1,6-diphosphate
-
 Neurospora crassa
1.1.1.44 p-hydroxymercuribenzoate
-
 Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.01
-
 6-phosphogluconate
-
 Neurospora crassa
1.1.1.44 0.013
-
 NADP+
-
 Rattus norvegicus
1.1.1.44 0.015
-
 NADP+
-
 Neurospora crassa
1.1.1.44 0.015
-
 NADP+
-
 Enterococcus faecalis
1.1.1.44 0.02
-
 6-phosphogluconate
-
 Homo sapiens
1.1.1.44 0.02
-
 NADP+ type I and II Cyberlindnera jadinii
1.1.1.44 0.024
-
 6-phosphogluconate
-
 Enterococcus faecalis
1.1.1.44 0.03
-
 NADP+
-
 Homo sapiens
1.1.1.44 0.03
-
 NADP+
-
 Neurospora crassa
1.1.1.44 0.031
-
 6-phosphogluconate type II Cyberlindnera jadinii
1.1.1.44 0.052
-
 6-phosphogluconate type I Cyberlindnera jadinii
1.1.1.44 0.071
-
 6-phosphogluconate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 50000
-
 2 * 50000, type I, SDS-PAGE Cyberlindnera jadinii
1.1.1.44 52000
-
 2 * 52000, SDS-PAGE Homo sapiens
1.1.1.44 52000
-
 2 * 52000, SDS-PAGE Rattus norvegicus
1.1.1.44 52000
-
 2 * 52000, SDS-PAGE Enterococcus faecalis
1.1.1.44 57000
-
 2 * 57000, SDS-PAGE Neurospora crassa
1.1.1.44 101000
-
 type I, centrifugation in sucrose density gradient Cyberlindnera jadinii
1.1.1.44 102000
-
-
 Rattus norvegicus
1.1.1.44 104000
-
-
 Homo sapiens
1.1.1.44 106000 115000 gel filtration Enterococcus faecalis
1.1.1.44 108000
-
 sedimentation equilibrium Enterococcus faecalis
1.1.1.44 110000 120000
-
 Neurospora crassa
1.1.1.44 111000
-
 type II Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Homo sapiens
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Rattus norvegicus
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Neurospora crassa
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Enterococcus faecalis
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Cyberlindnera jadinii
-
 type I, type II
-
1.1.1.44 Enterococcus faecalis
-
 ATCC 27792, formerly designated as strain MR
-
1.1.1.44 Homo sapiens
-
 human
-
1.1.1.44 Homo sapiens
-
 Krebs ascites cells
-
1.1.1.44 Neurospora crassa
-
-
-
1.1.1.44 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Cyberlindnera jadinii
1.1.1.44
-
 Homo sapiens
1.1.1.44
-
 Rattus norvegicus
1.1.1.44
-
 Neurospora crassa
1.1.1.44
-
 Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Homo sapiens
-
1.1.1.44 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 14.47
-
-
 Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
1.1.1.44 5°C, purified enzyme is stable to storage in 50 mM Tris HCl buffer, pH 7.5, 10 mM dithiothreitol and 20% glycerol, no significant decrease in activity occurs over a 2 week period Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Homo sapiens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Enterococcus faecalis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 D-ribulose 5-phosphate + CO2 + NADPH reverse reaction only to a very limited extent Enterococcus faecalis 6-phospho-D-gluconate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer 2 * 57000, SDS-PAGE Neurospora crassa
1.1.1.44 dimer 2 * 52000, SDS-PAGE Homo sapiens
1.1.1.44 dimer 2 * 52000, SDS-PAGE Rattus norvegicus
1.1.1.44 dimer 2 * 52000, SDS-PAGE Enterococcus faecalis
1.1.1.44 dimer 2 * 50000, type I, SDS-PAGE Cyberlindnera jadinii