EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.44 | synthesis | thermostability may lead to some practical applications | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii | |
1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | Ellman's reagent | Geobacillus stearothermophilus | |
1.1.1.44 | Hg2+ | - |
Geobacillus stearothermophilus | |
1.1.1.44 | p-chloromercuribenzoate | - |
Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.44 | 0.011 | - |
NADP+ | 60°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.014 | - |
6-phosphogluconate | 60°C or 65°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.015 | - |
NADP+ | 65°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.016 | - |
6-phosphogluconate | 30°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.017 | - |
NADP+ | 30°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.02 | - |
6-phosphogluconate | 43°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.021 | - |
NADP+ | 45°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.022 | - |
6-phosphogluconate | 45°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.025 | - |
NADP+ | 43°C | Geobacillus stearothermophilus | |
1.1.1.44 | 0.55 | - |
2-deoxy-6-phosphogluconate | - |
Cyberlindnera jadinii | |
1.1.1.44 | 1 | - |
NAD+ | 43°C | Cyberlindnera jadinii | |
1.1.1.44 | 3.5 | - |
NAD+ | 30°C | Cyberlindnera jadinii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | KCl | activates the enzyme at high concentrations | Geobacillus stearothermophilus | |
1.1.1.44 | Mg2+ | - |
Cyberlindnera jadinii | |
1.1.1.44 | Mg2+ | activates the enzyme at low concentrations 0.01-0.04 M | Geobacillus stearothermophilus | |
1.1.1.44 | NaCl | activates the enzyme at high concentrations | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 51000 | - |
2 * 51000, SDS-PAGE | Geobacillus stearothermophilus |
1.1.1.44 | 101000 | - |
- |
Cyberlindnera jadinii |
1.1.1.44 | 101000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Homo sapiens | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Rattus norvegicus | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Neurospora crassa | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus NCA 1503 | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.44 | Cyberlindnera jadinii | - |
- |
- |
1.1.1.44 | Geobacillus stearothermophilus | - |
NCA 1503 | - |
1.1.1.44 | Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
1.1.1.44 | Homo sapiens | - |
human | - |
1.1.1.44 | Neurospora crassa | - |
- |
- |
1.1.1.44 | Rattus norvegicus | - |
rat | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.44 | - |
Geobacillus stearothermophilus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.44 | erythrocyte | - |
Homo sapiens | - |
1.1.1.44 | liver | - |
Homo sapiens | - |
1.1.1.44 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 87 | - |
- |
Geobacillus stearothermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.44 | 4°C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity | Cyberlindnera jadinii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NAD+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADH | - |
? | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Homo sapiens | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Rattus norvegicus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.44 | dimer | - |
Cyberlindnera jadinii |
1.1.1.44 | dimer | - |
Homo sapiens |
1.1.1.44 | dimer | - |
Rattus norvegicus |
1.1.1.44 | dimer | - |
Neurospora crassa |
1.1.1.44 | dimer | 2 * 51000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 60 | - |
stable at 60°C | Geobacillus stearothermophilus |
1.1.1.44 | 70 | - |
denaturation half-time of 80 min | Geobacillus stearothermophilus |
1.1.1.44 | 80 | - |
denaturation half-time of 3 min | Geobacillus stearothermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 8 | - |
- |
Cyberlindnera jadinii |
1.1.1.44 | 8 | - |
- |
Homo sapiens |
1.1.1.44 | 8 | - |
- |
Rattus norvegicus |
1.1.1.44 | 8 | - |
- |
Geobacillus stearothermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | NAD+ | - |
Cyberlindnera jadinii | |
1.1.1.44 | NAD+ | - |
Rattus norvegicus | |
1.1.1.44 | NAD+ | some activity with NAD+ | Geobacillus stearothermophilus | |
1.1.1.44 | NADP+ | - |
Cyberlindnera jadinii | |
1.1.1.44 | NADP+ | - |
Rattus norvegicus | |
1.1.1.44 | NADP+ | - |
Geobacillus stearothermophilus | |
1.1.1.44 | NADP+ | - |
Neurospora crassa |