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Literature summary extracted from

  • Veronese, F.M.; Boccu, E.; Fontana, A.; Benassi, C.A.; Scoffone, E.
    Isolation and some properties of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus (1974), Biochim. Biophys. Acta, 334, 31-44.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.44 synthesis thermostability may lead to some practical applications Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 5,5'-dithiobis(2-nitrobenzoic acid)
-
Cyberlindnera jadinii
1.1.1.44 5,5'-dithiobis(2-nitrobenzoic acid) Ellman's reagent Geobacillus stearothermophilus
1.1.1.44 Hg2+
-
Geobacillus stearothermophilus
1.1.1.44 p-chloromercuribenzoate
-
Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.011
-
NADP+ 60°C Geobacillus stearothermophilus
1.1.1.44 0.014
-
6-phosphogluconate 60°C or 65°C Geobacillus stearothermophilus
1.1.1.44 0.015
-
NADP+ 65°C Geobacillus stearothermophilus
1.1.1.44 0.016
-
6-phosphogluconate 30°C Geobacillus stearothermophilus
1.1.1.44 0.017
-
NADP+ 30°C Geobacillus stearothermophilus
1.1.1.44 0.02
-
6-phosphogluconate 43°C Geobacillus stearothermophilus
1.1.1.44 0.021
-
NADP+ 45°C Geobacillus stearothermophilus
1.1.1.44 0.022
-
6-phosphogluconate 45°C Geobacillus stearothermophilus
1.1.1.44 0.025
-
NADP+ 43°C Geobacillus stearothermophilus
1.1.1.44 0.55
-
2-deoxy-6-phosphogluconate
-
Cyberlindnera jadinii
1.1.1.44 1
-
NAD+ 43°C Cyberlindnera jadinii
1.1.1.44 3.5
-
NAD+ 30°C Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.44 KCl activates the enzyme at high concentrations Geobacillus stearothermophilus
1.1.1.44 Mg2+
-
Cyberlindnera jadinii
1.1.1.44 Mg2+ activates the enzyme at low concentrations 0.01-0.04 M Geobacillus stearothermophilus
1.1.1.44 NaCl activates the enzyme at high concentrations Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 51000
-
2 * 51000, SDS-PAGE Geobacillus stearothermophilus
1.1.1.44 101000
-
-
Cyberlindnera jadinii
1.1.1.44 101000
-
sucrose density gradient centrifugation Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+ Homo sapiens
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+ Rattus norvegicus
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+ Neurospora crassa
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus NCA 1503
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Cyberlindnera jadinii
-
-
-
1.1.1.44 Geobacillus stearothermophilus
-
NCA 1503
-
1.1.1.44 Geobacillus stearothermophilus NCA 1503
-
NCA 1503
-
1.1.1.44 Homo sapiens
-
human
-
1.1.1.44 Neurospora crassa
-
-
-
1.1.1.44 Rattus norvegicus
-
rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
Geobacillus stearothermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 erythrocyte
-
Homo sapiens
-
1.1.1.44 liver
-
Homo sapiens
-
1.1.1.44 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 87
-
-
Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
1.1.1.44 4°C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NAD+
-
Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADH
-
?
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Homo sapiens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
Geobacillus stearothermophilus NCA 1503 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer
-
Cyberlindnera jadinii
1.1.1.44 dimer
-
Homo sapiens
1.1.1.44 dimer
-
Rattus norvegicus
1.1.1.44 dimer
-
Neurospora crassa
1.1.1.44 dimer 2 * 51000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.44 60
-
stable at 60°C Geobacillus stearothermophilus
1.1.1.44 70
-
denaturation half-time of 80 min Geobacillus stearothermophilus
1.1.1.44 80
-
denaturation half-time of 3 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 8
-
-
Cyberlindnera jadinii
1.1.1.44 8
-
-
Homo sapiens
1.1.1.44 8
-
-
Rattus norvegicus
1.1.1.44 8
-
-
Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NAD+
-
Cyberlindnera jadinii
1.1.1.44 NAD+
-
Rattus norvegicus
1.1.1.44 NAD+ some activity with NAD+ Geobacillus stearothermophilus
1.1.1.44 NADP+
-
Cyberlindnera jadinii
1.1.1.44 NADP+
-
Rattus norvegicus
1.1.1.44 NADP+
-
Geobacillus stearothermophilus
1.1.1.44 NADP+
-
Neurospora crassa