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Literature summary extracted from
- Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus (1997), Arch. MIcrobiol., 168, 412-420.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.42 | 0.03 | - |
NADP+ | - |
Archaeoglobus fulgidus |  |
| 1.1.1.42 | 0.118 | - |
D,L-isocitrate | - |
Archaeoglobus fulgidus | |
| 1.1.1.42 | 5.4 | - |
NAD+ | - |
Archaeoglobus fulgidus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.42 | Mg2+ | absolute requirement for divalent cations | Archaeoglobus fulgidus | |
| 1.1.1.42 | Mn2+ | absolute requirement for divalent cations | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 42000 | - |
alpha2, 2 * 42000, SDS-PAGE | Archaeoglobus fulgidus |
| 1.1.1.42 | 80000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NADP+ | Archaeoglobus fulgidus | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.42 | Archaeoglobus fulgidus | O29610 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.42 | - |
Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 141 | - |
- |
Archaeoglobus fulgidus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.42 | 4°C, several months, under oxic conditions | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.42 | isocitrate + NAD+ | - |
Archaeoglobus fulgidus | 2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
| 1.1.1.42 | isocitrate + NADP+ | - |
Archaeoglobus fulgidus | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.42 | dimer | alpha2, 2 * 42000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 90 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 90 | - |
half life of 22 min in 50 mM tricine-KOH buffer, pH 8 | Archaeoglobus fulgidus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.42 | 8.6 | - |
- |
Archaeoglobus fulgidus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.42 | NAD+ | - |
Archaeoglobus fulgidus | |
| 1.1.1.42 | NADP+ | - |
Archaeoglobus fulgidus | |
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Release 2023.1 (January 2023)
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