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Literature summary extracted from

  • Hsu, R.Y.; Glynias, M.J.; Satterlee, J.; Feeney, R.; Clarke, A.R.; Emery, D.C.; Roe, B.A.; Wilson, R.K.; Goodridge, A.G.; Holbrook, J.J.
    Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants (1992), Biochem. J., 284, 869-876.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expression in Escherichia coli TH-2 cells as a fusion protein including a 15-residue N-terminal leader from beta-galactosidase coded by the lacZ gene Anas platyrhynchos

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.40 C99S turnover number decreases 3fold and Km for malate increases 4fold Anas platyrhynchos
1.1.1.40 R70Q kinetic parameters are similar except for a slightly lower turnover number and higher Km for L-malate Anas platyrhynchos

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.0046
-
 NADP+
-
 Anas platyrhynchos
1.1.1.40 0.073
-
 L-malate
-
 Anas platyrhynchos

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Anas platyrhynchos
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40
-
 Anas platyrhynchos

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 liver
-
 Anas platyrhynchos
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Anas platyrhynchos pyruvate + CO2 + NADPH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 160
-
 L-malate
-
 Anas platyrhynchos

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+ cofactor Anas platyrhynchos