BRENDA - Enzyme Database show

Purification and properties of pigeon liver malic enzyme

Rutter, W.J.; Lardy, H.A.; J. Biol. Chem. 233, 374-382 (1958)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.40
malic acid decreases heat stability at pH values above 5.0
Pigeon
1.1.1.40
Mg2+ or Mn2+ increase heat stability
Pigeon
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.40
adenosine 2'-phosphate
-
Pigeon
1.1.1.40
ADP
-
Pigeon
1.1.1.40
arsenite
weak
Pigeon
1.1.1.40
Cu2+
3 mM, weak
Pigeon
1.1.1.40
diphenyliodonium chloride
weak
Pigeon
1.1.1.40
Hg2+
0.0005 mM, almost complete inhibition
Pigeon
1.1.1.40
iodoacetate
weak
Pigeon
1.1.1.40
NAD+
weak
Pigeon
1.1.1.40
o-Iodosobenzoate
strong
Pigeon
1.1.1.40
p-mercuribenzoate
strong
Pigeon
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.40
0.016
-
NADP+
pH 7.5
Pigeon
1.1.1.40
0.041
-
L-malate
pH 6.5
Pigeon
1.1.1.40
0.39
-
L-malate
pH 7.5
Pigeon
1.1.1.40
3.3
-
L-malate
pH 8.5
Pigeon
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.40
Co2+
less effective activation than Mn2+
Pigeon
1.1.1.40
Cu2+
weak activation
Pigeon
1.1.1.40
Mg2+
promotes activity
Pigeon
1.1.1.40
Mn2+
0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase
Pigeon
1.1.1.40
Ni2+
weak activation
Pigeon
1.1.1.40
Zn2+
weak activation
Pigeon
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.40
Pigeon
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.40
-
Pigeon
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.40
liver
-
Pigeon
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.40
additional information
-
-
Pigeon
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.40
(S)-malate + NADP+
the singly-ionized species is the substrate, doubly-ionized malate is unreactive
286722
Pigeon
pyruvate + CO2 + NADPH
-
286722
Pigeon
r
1.1.1.40
Oxaloacetate
-
286722
Pigeon
Pyruvate + CO2
-
286722
Pigeon
?
1.1.1.40
pyruvate + CO2 + NADPH
-
286722
Pigeon
L-malate + NADP+
-
286722
Pigeon
r
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.40
additional information
-
malic acid decreases heat stability at pH values above 5.0; Mg2+ or Mn2+ increase heat stability
Pigeon
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.40
7.2
-
in presence of 0.1 mM malate
Pigeon
1.1.1.40
8.5
9
in presence of 0.1 M malate
Pigeon
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.40
NADP+
cofactor; stimulation of decarboxylation of oxaloacetate
Pigeon
1.1.1.40
NADPH
cofactor
Pigeon
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.40
NADP+
cofactor; stimulation of decarboxylation of oxaloacetate
Pigeon
1.1.1.40
NADPH
cofactor
Pigeon
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.40
malic acid decreases heat stability at pH values above 5.0
Pigeon
1.1.1.40
Mg2+ or Mn2+ increase heat stability
Pigeon
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.40
adenosine 2'-phosphate
-
Pigeon
1.1.1.40
ADP
-
Pigeon
1.1.1.40
arsenite
weak
Pigeon
1.1.1.40
Cu2+
3 mM, weak
Pigeon
1.1.1.40
diphenyliodonium chloride
weak
Pigeon
1.1.1.40
Hg2+
0.0005 mM, almost complete inhibition
Pigeon
1.1.1.40
iodoacetate
weak
Pigeon
1.1.1.40
NAD+
weak
Pigeon
1.1.1.40
o-Iodosobenzoate
strong
Pigeon
1.1.1.40
p-mercuribenzoate
strong
Pigeon
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.40
0.016
-
NADP+
pH 7.5
Pigeon
1.1.1.40
0.041
-
L-malate
pH 6.5
Pigeon
1.1.1.40
0.39
-
L-malate
pH 7.5
Pigeon
1.1.1.40
3.3
-
L-malate
pH 8.5
Pigeon
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.40
Co2+
less effective activation than Mn2+
Pigeon
1.1.1.40
Cu2+
weak activation
Pigeon
1.1.1.40
Mg2+
promotes activity
Pigeon
1.1.1.40
Mn2+
0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase
Pigeon
1.1.1.40
Ni2+
weak activation
Pigeon
1.1.1.40
Zn2+
weak activation
Pigeon
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.40
-
Pigeon
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.40
liver
-
Pigeon
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.40
additional information
-
-
Pigeon
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.40
(S)-malate + NADP+
the singly-ionized species is the substrate, doubly-ionized malate is unreactive
286722
Pigeon
pyruvate + CO2 + NADPH
-
286722
Pigeon
r
1.1.1.40
Oxaloacetate
-
286722
Pigeon
Pyruvate + CO2
-
286722
Pigeon
?
1.1.1.40
pyruvate + CO2 + NADPH
-
286722
Pigeon
L-malate + NADP+
-
286722
Pigeon
r
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.40
additional information
-
malic acid decreases heat stability at pH values above 5.0; Mg2+ or Mn2+ increase heat stability
Pigeon
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.40
7.2
-
in presence of 0.1 mM malate
Pigeon
1.1.1.40
8.5
9
in presence of 0.1 M malate
Pigeon