BRENDA - Enzyme Database

Purification and characterization of an NAD-malic enzyme from Bradyrhizobium japonicum A1017

Chen, F.; Okabe, Y.; Osano, K.; Tajima, S.; Appl. Environ. Microbiol. 64, 4073-4075 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.39
adenosine 5'-diphosphoglucose
activates
Bradyrhizobium japonicum
1.1.1.39
alpha-D-glucose 1-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
D-fructose 6-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
D-glucose 6-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
fructose 1,6-bisphosphate
activates
Bradyrhizobium japonicum
1.1.1.39
gamma-amino-n-butyrate
slight activation
Bradyrhizobium japonicum
1.1.1.39
hydroxy-n-butyrate
slight activation
Bradyrhizobium japonicum
1.1.1.39
Hydroxypyruvate
slight activation
Bradyrhizobium japonicum
1.1.1.39
succinate
stimulates
Bradyrhizobium japonicum
1.1.1.39
uridine 5'-diphosphoglucose
activates
Bradyrhizobium japonicum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.39
2-Ketoglutarate
-
Bradyrhizobium japonicum
1.1.1.39
AMP
-
Bradyrhizobium japonicum
1.1.1.39
ATP
-
Bradyrhizobium japonicum
1.1.1.39
CoA
-
Bradyrhizobium japonicum
1.1.1.39
DL-isocitrate
-
Bradyrhizobium japonicum
1.1.1.39
fumarate
-
Bradyrhizobium japonicum
1.1.1.39
L-asparatate
-
Bradyrhizobium japonicum
1.1.1.39
malonate
-
Bradyrhizobium japonicum
1.1.1.39
phosphoenolpyruvate
-
Bradyrhizobium japonicum
1.1.1.39
pyruvate
-
Bradyrhizobium japonicum
1.1.1.39
Tartrate
-
Bradyrhizobium japonicum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.39
Mg2+
strict requirement for a divalent cation, maximal activation at 2 mM
Bradyrhizobium japonicum
1.1.1.39
Mn2+
strict requirement for a divalent cation, maximal activation at 5 mM
Bradyrhizobium japonicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.39
85000
-
4 * 85000, SDS-PAGE
Bradyrhizobium japonicum
1.1.1.39
388000
-
gel filtration
Bradyrhizobium japonicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.39
Bradyrhizobium japonicum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.39
-
Bradyrhizobium japonicum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.39
additional information
-
-
Bradyrhizobium japonicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.39
malate + NAD+
-
286720
Bradyrhizobium japonicum
pyruvate + CO2 + NADH
-
286720
Bradyrhizobium japonicum
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.39
tetramer
4 * 85000, SDS-PAGE
Bradyrhizobium japonicum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.39
NAD+
cofactor
Bradyrhizobium japonicum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.39
adenosine 5'-diphosphoglucose
activates
Bradyrhizobium japonicum
1.1.1.39
alpha-D-glucose 1-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
D-fructose 6-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
D-glucose 6-phosphate
activation
Bradyrhizobium japonicum
1.1.1.39
fructose 1,6-bisphosphate
activates
Bradyrhizobium japonicum
1.1.1.39
gamma-amino-n-butyrate
slight activation
Bradyrhizobium japonicum
1.1.1.39
hydroxy-n-butyrate
slight activation
Bradyrhizobium japonicum
1.1.1.39
Hydroxypyruvate
slight activation
Bradyrhizobium japonicum
1.1.1.39
succinate
stimulates
Bradyrhizobium japonicum
1.1.1.39
uridine 5'-diphosphoglucose
activates
Bradyrhizobium japonicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.39
NAD+
cofactor
Bradyrhizobium japonicum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.39
2-Ketoglutarate
-
Bradyrhizobium japonicum
1.1.1.39
AMP
-
Bradyrhizobium japonicum
1.1.1.39
ATP
-
Bradyrhizobium japonicum
1.1.1.39
CoA
-
Bradyrhizobium japonicum
1.1.1.39
DL-isocitrate
-
Bradyrhizobium japonicum
1.1.1.39
fumarate
-
Bradyrhizobium japonicum
1.1.1.39
L-asparatate
-
Bradyrhizobium japonicum
1.1.1.39
malonate
-
Bradyrhizobium japonicum
1.1.1.39
phosphoenolpyruvate
-
Bradyrhizobium japonicum
1.1.1.39
pyruvate
-
Bradyrhizobium japonicum
1.1.1.39
Tartrate
-
Bradyrhizobium japonicum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.39
Mg2+
strict requirement for a divalent cation, maximal activation at 2 mM
Bradyrhizobium japonicum
1.1.1.39
Mn2+
strict requirement for a divalent cation, maximal activation at 5 mM
Bradyrhizobium japonicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.39
85000
-
4 * 85000, SDS-PAGE
Bradyrhizobium japonicum
1.1.1.39
388000
-
gel filtration
Bradyrhizobium japonicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.39
-
Bradyrhizobium japonicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.39
additional information
-
-
Bradyrhizobium japonicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.39
malate + NAD+
-
286720
Bradyrhizobium japonicum
pyruvate + CO2 + NADH
-
286720
Bradyrhizobium japonicum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.39
tetramer
4 * 85000, SDS-PAGE
Bradyrhizobium japonicum