Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suye, S.; Okada, Y.; Funada, A.; Kawagoe, M.; Inuta, S.
    Purification and properties of malic enzyme from Pseudomonas diminuta IFO-13182 (1992), J. Ferment. Bioeng., 73, 343-347.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 CuSO4
-
 Brevundimonas diminuta
1.1.1.39 EDTA
-
 Brevundimonas diminuta
1.1.1.39 HgCl2
-
 Brevundimonas diminuta
1.1.1.39 hydroquinone
-
 Brevundimonas diminuta
1.1.1.39 NEM
-
 Brevundimonas diminuta
1.1.1.39 oxalate
-
 Brevundimonas diminuta
1.1.1.39 PCMB
-
 Brevundimonas diminuta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 5.97
-
 (S)-malate with NAD+ as coenzyme Brevundimonas diminuta
1.1.1.39 8.34
-
 (S)-malate with NADP+ as coenzyme Brevundimonas diminuta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ divalent metal ion required, NAD+-linked activity shows maximal activity at 5 mM Mg2+ Brevundimonas diminuta
1.1.1.39 Ni2+ divalent metal ion required, NADP+-linked activity exhibits a maximum at 5 mM Ni2+ Brevundimonas diminuta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 64000
-
 10 * 64000, SDS-PAGE Brevundimonas diminuta
1.1.1.39 680000
-
 gel filtration Brevundimonas diminuta

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Brevundimonas diminuta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Brevundimonas diminuta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 2.74
-
 NAD+-linked activity Brevundimonas diminuta
1.1.1.39 5.45
-
 NADP+-linked activity Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 malate + NAD+
-
 Brevundimonas diminuta pyruvate + CO2 + NADH + H+
-
 ?
1.1.1.39 additional information no decarboxylation of oxaloacetate Brevundimonas diminuta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.39 decamer 10 * 64000, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.39 50
-
-
 Brevundimonas diminuta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.39 30 60 30°C: about 35% of maximal activity, 60°C: about 50% of maximal activity Brevundimonas diminuta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.39 45
-
 15 min, stable at or below Brevundimonas diminuta
1.1.1.39 55
-
 15 min, complete inactivation Brevundimonas diminuta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 7 8
-
 Brevundimonas diminuta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.39 6 8.5 about 40% of maximal activity at pH 6.0 and at pH 8.5 Brevundimonas diminuta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.39 7 9 25°C, 5 min, stable Brevundimonas diminuta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Brevundimonas diminuta
1.1.1.39 NADP+ activity with NADP+ is about twice that measured with NAD+ Brevundimonas diminuta