BRENDA - Enzyme Database

Characterization of two members of a novel malic enzyme class

Voegele, R.T.; Mitsch, M.J.; Finan, T.M.; Biochim. Biophys. Acta 1432, 275-285 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.39
ADP
slight stimulstion
Sinorhizobium meliloti
1.1.1.39
aspartate
stimulates
Sinorhizobium meliloti
1.1.1.39
ATP
slight stimulstion
Sinorhizobium meliloti
1.1.1.39
fructose 1,6-bisphosphate
stimulates
Sinorhizobium meliloti
1.1.1.39
fumarate
stimulates
Sinorhizobium meliloti
1.1.1.39
succinate
stimulates
Sinorhizobium meliloti
General Stability
EC Number
General Stability
Organism
1.1.1.40
loss of activity upon repeated freezing and thawing
Sinorhizobium meliloti
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.39
acetyl-CoA
potent inhibitor
Sinorhizobium meliloti
1.1.1.39
bicarbonate
-
Sinorhizobium meliloti
1.1.1.39
D-Tartrate
-
Sinorhizobium meliloti
1.1.1.39
DL-lactate
-
Sinorhizobium meliloti
1.1.1.39
glyoxylate
-
Sinorhizobium meliloti
1.1.1.39
malonate
-
Sinorhizobium meliloti
1.1.1.39
NADH
-
Sinorhizobium meliloti
1.1.1.39
oxaloacetate
-
Sinorhizobium meliloti
1.1.1.39
phosphoenolpyruvate
-
Sinorhizobium meliloti
1.1.1.39
pyruvate
-
Sinorhizobium meliloti
1.1.1.39
Tartronate
-
Sinorhizobium meliloti
1.1.1.40
D-malate
-
Sinorhizobium meliloti
1.1.1.40
D-Tartrate
-
Sinorhizobium meliloti
1.1.1.40
glyoxylate
-
Sinorhizobium meliloti
1.1.1.40
L-Tartrate
-
Sinorhizobium meliloti
1.1.1.40
malonate
-
Sinorhizobium meliloti
1.1.1.40
Tartronate
-
Sinorhizobium meliloti
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.39
0.089
-
NAD+
-
Sinorhizobium meliloti
1.1.1.39
1.56
-
NADP+
-
Sinorhizobium meliloti
1.1.1.40
2.6
-
L-malate
-
Sinorhizobium meliloti
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.39
82000
-
x * 82000, SDS-PAGE
Sinorhizobium meliloti
1.1.1.40
82000
-
x * 82000, SDS-PAGE
Sinorhizobium meliloti
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.39
Sinorhizobium meliloti
-
-
-
1.1.1.40
Sinorhizobium meliloti
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.39
-
Sinorhizobium meliloti
1.1.1.40
-
Sinorhizobium meliloti
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.39
45.2
-
-
Sinorhizobium meliloti
1.1.1.40
49
-
-
Sinorhizobium meliloti
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.39
-20°C, stable for several weeks
Sinorhizobium meliloti
1.1.1.39
4°C, loss of activity upon prolonged storage
Sinorhizobium meliloti
1.1.1.40
-20°C, stable for several weeks
Sinorhizobium meliloti
1.1.1.40
4°C, loss of activity upon prolonged storage
Sinorhizobium meliloti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.39
malate + NAD+
-
286706
Sinorhizobium meliloti
pyruvate + CO2 + NADH
-
286706
Sinorhizobium meliloti
ir
1.1.1.39
additional information
no decarboxylation of oxaloacetate
286706
Sinorhizobium meliloti
?
-
-
-
-
1.1.1.40
(S)-malate + NADP+
-
286706
Sinorhizobium meliloti
pyruvate + CO2 + NADPH
-
-
-
ir
1.1.1.40
Oxaloacetate
no activity
286706
Sinorhizobium meliloti
Pyruvate + CO2
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.39
?
x * 82000, SDS-PAGE
Sinorhizobium meliloti
1.1.1.40
?
x * 82000, SDS-PAGE
Sinorhizobium meliloti
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.40
582
-
L-malate
assuming an octameric oligomerization state
Sinorhizobium meliloti
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.39
NAD+
cofactor
Sinorhizobium meliloti
1.1.1.39
NADP+
cofactor
Sinorhizobium meliloti
1.1.1.40
NADP+
-
Sinorhizobium meliloti
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.39
ADP
slight stimulstion
Sinorhizobium meliloti
1.1.1.39
aspartate
stimulates
Sinorhizobium meliloti
1.1.1.39
ATP
slight stimulstion
Sinorhizobium meliloti
1.1.1.39
fructose 1,6-bisphosphate
stimulates
Sinorhizobium meliloti
1.1.1.39
fumarate
stimulates
Sinorhizobium meliloti
1.1.1.39
succinate
stimulates
Sinorhizobium meliloti
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.39
NAD+
cofactor
Sinorhizobium meliloti
1.1.1.39
NADP+
cofactor
Sinorhizobium meliloti
1.1.1.40
NADP+
-
Sinorhizobium meliloti
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.40
loss of activity upon repeated freezing and thawing
Sinorhizobium meliloti
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.39
acetyl-CoA
potent inhibitor
Sinorhizobium meliloti
1.1.1.39
bicarbonate
-
Sinorhizobium meliloti
1.1.1.39
D-Tartrate
-
Sinorhizobium meliloti
1.1.1.39
DL-lactate
-
Sinorhizobium meliloti
1.1.1.39
glyoxylate
-
Sinorhizobium meliloti
1.1.1.39
malonate
-
Sinorhizobium meliloti
1.1.1.39
NADH
-
Sinorhizobium meliloti
1.1.1.39
oxaloacetate
-
Sinorhizobium meliloti
1.1.1.39
phosphoenolpyruvate
-
Sinorhizobium meliloti
1.1.1.39
pyruvate
-
Sinorhizobium meliloti
1.1.1.39
Tartronate
-
Sinorhizobium meliloti
1.1.1.40
D-malate
-
Sinorhizobium meliloti
1.1.1.40
D-Tartrate
-
Sinorhizobium meliloti
1.1.1.40
glyoxylate
-
Sinorhizobium meliloti
1.1.1.40
L-Tartrate
-
Sinorhizobium meliloti
1.1.1.40
malonate
-
Sinorhizobium meliloti
1.1.1.40
Tartronate
-
Sinorhizobium meliloti
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.39
0.089
-
NAD+
-
Sinorhizobium meliloti
1.1.1.39
1.56
-
NADP+
-
Sinorhizobium meliloti
1.1.1.40
2.6
-
L-malate
-
Sinorhizobium meliloti
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.39
82000
-
x * 82000, SDS-PAGE
Sinorhizobium meliloti
1.1.1.40
82000
-
x * 82000, SDS-PAGE
Sinorhizobium meliloti
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.39
-
Sinorhizobium meliloti
1.1.1.40
-
Sinorhizobium meliloti
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.39
45.2
-
-
Sinorhizobium meliloti
1.1.1.40
49
-
-
Sinorhizobium meliloti
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.39
-20°C, stable for several weeks
Sinorhizobium meliloti
1.1.1.39
4°C, loss of activity upon prolonged storage
Sinorhizobium meliloti
1.1.1.40
-20°C, stable for several weeks
Sinorhizobium meliloti
1.1.1.40
4°C, loss of activity upon prolonged storage
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.39
malate + NAD+
-
286706
Sinorhizobium meliloti
pyruvate + CO2 + NADH
-
286706
Sinorhizobium meliloti
ir
1.1.1.39
additional information
no decarboxylation of oxaloacetate
286706
Sinorhizobium meliloti
?
-
-
-
-
1.1.1.40
(S)-malate + NADP+
-
286706
Sinorhizobium meliloti
pyruvate + CO2 + NADPH
-
-
-
ir
1.1.1.40
Oxaloacetate
no activity
286706
Sinorhizobium meliloti
Pyruvate + CO2
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.39
?
x * 82000, SDS-PAGE
Sinorhizobium meliloti
1.1.1.40
?
x * 82000, SDS-PAGE
Sinorhizobium meliloti
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.40
582
-
L-malate
assuming an octameric oligomerization state
Sinorhizobium meliloti