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Literature summary extracted from

  • Van Kuijk, B.L.M.; Stams, A.J.M.
    Purification and characterization of malate dehydrogenase from the syntrophic propionate-oxidizing bacterium strain MPOB (1996), FEMS Microbiol. Lett., 144, 141-144.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 oxaloacetate
-
 Syntrophobacter fumaroxidans
1.1.1.37 oxaloacetate
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.03
-
 NADH
-
 Syntrophobacter fumaroxidans
1.1.1.37 0.05
-
 oxaloacetate
-
 Syntrophobacter fumaroxidans
1.1.1.37 1.1
-
 NAD+
-
 Syntrophobacter fumaroxidans
1.1.1.37 4
-
 L-malate
-
 Syntrophobacter fumaroxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytoplasm
-
 Mus musculus 5737
-
1.1.1.37 mitochondrion
-
 Mus musculus 5739
-
1.1.1.37 mitochondrion
-
 Thermus thermophilus 5739
-
1.1.1.37 mitochondrion
-
 Citrullus lanatus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 35000
-
 2 * 35000, SDS-PAGE Syntrophobacter fumaroxidans
1.1.1.37 68000
-
 gel filtration Syntrophobacter fumaroxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Bacillus subtilis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Mus musculus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Thermus thermophilus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Geobacillus stearothermophilus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Methanothermus fervidus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Streptosporangium roseum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Citrullus lanatus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Chlorobaculum tepidum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Syntrophobacter fumaroxidans
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Planomonospora venezuelensis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Streptomyces atratus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Phenylobacterium immobile
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Prosthecochloris vibrioformis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Heliomicrobium gestii
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Syntrophobacter fumaroxidans MPOB
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Bacillus subtilis
-
-
-
1.1.1.37 Chlorobaculum tepidum
-
-
-
1.1.1.37 Citrullus lanatus
-
 watermelon
-
1.1.1.37 Escherichia coli
-
-
-
1.1.1.37 Geobacillus stearothermophilus
-
-
-
1.1.1.37 Heliomicrobium gestii
-
-
-
1.1.1.37 Methanothermus fervidus
-
-
-
1.1.1.37 Mus musculus
-
 mouse
-
1.1.1.37 Phenylobacterium immobile
-
-
-
1.1.1.37 Planomonospora venezuelensis
-
-
-
1.1.1.37 Prosthecochloris vibrioformis
-
-
-
1.1.1.37 Streptomyces atratus
-
-
-
1.1.1.37 Streptosporangium roseum
-
-
-
1.1.1.37 Syntrophobacter fumaroxidans
-
 DSM 10017
-
1.1.1.37 Syntrophobacter fumaroxidans MPOB
-
 DSM 10017
-
1.1.1.37 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Mus musculus
1.1.1.37
-
 Thermus thermophilus
1.1.1.37
-
 Escherichia coli
1.1.1.37
-
 Streptosporangium roseum
1.1.1.37
-
 Citrullus lanatus
1.1.1.37
-
 Chlorobaculum tepidum
1.1.1.37
-
 Syntrophobacter fumaroxidans
1.1.1.37
-
 Planomonospora venezuelensis
1.1.1.37
-
 Streptomyces atratus
1.1.1.37
-
 Prosthecochloris vibrioformis
1.1.1.37
-
 Heliomicrobium gestii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 1728
-
-
 Syntrophobacter fumaroxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Mus musculus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Thermus thermophilus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Methanothermus fervidus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Streptosporangium roseum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Chlorobaculum tepidum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Syntrophobacter fumaroxidans oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Planomonospora venezuelensis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Streptomyces atratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Phenylobacterium immobile oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Prosthecochloris vibrioformis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Heliomicrobium gestii oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Syntrophobacter fumaroxidans MPOB oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Mus musculus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thermus thermophilus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Methanothermus fervidus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Streptosporangium roseum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Chlorobaculum tepidum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Syntrophobacter fumaroxidans oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Planomonospora venezuelensis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Streptomyces atratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Phenylobacterium immobile oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Prosthecochloris vibrioformis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Heliomicrobium gestii oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Syntrophobacter fumaroxidans MPOB oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bacillus subtilis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Mus musculus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thermus thermophilus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Geobacillus stearothermophilus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Methanothermus fervidus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Streptosporangium roseum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Citrullus lanatus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Chlorobaculum tepidum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Syntrophobacter fumaroxidans L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Planomonospora venezuelensis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Streptomyces atratus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Phenylobacterium immobile L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Prosthecochloris vibrioformis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Heliomicrobium gestii L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Syntrophobacter fumaroxidans MPOB L-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer
-
 Thermus thermophilus
1.1.1.37 dimer 2 * 35000, SDS-PAGE Syntrophobacter fumaroxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 60
-
-
 Syntrophobacter fumaroxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.5
-
 reduction of oxaloacetate Syntrophobacter fumaroxidans
1.1.1.37 8.5
-
-
 Syntrophobacter fumaroxidans
1.1.1.37 9
-
 oxidation of L-malate Syntrophobacter fumaroxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Syntrophobacter fumaroxidans
1.1.1.37 NADP+ low activity with Syntrophobacter fumaroxidans