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Literature summary extracted from

  • Smith, K.; Sundaram, T.K.; Kernick, M.; Wilkinson, A.E.
    Purification of bacterial malate dehydrogenases by selective elution from a triazinyl dye affinity column (1982), Biochim. Biophys. Acta, 708, 17-25.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Thermus aquaticus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Bacillus subtilis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Bacillus sp. (in: Bacteria)
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ [Bacillus] caldotenax
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Comamonas testosteroni
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Thermus aquaticus B
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Bacillus sp. (in: Bacteria)
-
 moderate thermophile, designated BI
-
1.1.1.37 Bacillus subtilis
-
-
-
1.1.1.37 Comamonas testosteroni
-
-
-
1.1.1.37 Escherichia coli
-
-
-
1.1.1.37 Thermus aquaticus
-
-
-
1.1.1.37 Thermus aquaticus B
-
-
-
1.1.1.37 [Bacillus] caldotenax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Thermus aquaticus
1.1.1.37
-
 Bacillus subtilis
1.1.1.37
-
 Escherichia coli
1.1.1.37
-
 [Bacillus] caldotenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 83
-
-
 [Bacillus] caldotenax
1.1.1.37 100.5
-
-
 Thermus aquaticus
1.1.1.37 187
-
-
 Bacillus sp. (in: Bacteria)
1.1.1.37 322
-
-
 Bacillus subtilis
1.1.1.37 1920
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 [Bacillus] caldotenax oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Comamonas testosteroni oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Thermus aquaticus B oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 [Bacillus] caldotenax oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Comamonas testosteroni oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thermus aquaticus B oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thermus aquaticus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bacillus subtilis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bacillus sp. (in: Bacteria) L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 [Bacillus] caldotenax L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Comamonas testosteroni L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thermus aquaticus B L-malate + NAD+
-
 r