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Literature summary extracted from

  • Iijima, S.; Saiki, T.; Beppu, T.
    Physicochemical and catalytic properties of thermostable malate dehydrogenase from an extreme thermophile Thermus flavus AT-62 (1980), Biochim. Biophys. Acta, 613, 1-9.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.37 remarkably resistant to urea and SDS Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 diethyldicarbonate
-
Thermus thermophilus
1.1.1.37 oxaloacetate
-
Comamonas testosteroni
1.1.1.37 oxaloacetate
-
Sus scrofa
1.1.1.37 oxaloacetate substrate inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.006
-
oxaloacetate 30°C Thermus thermophilus
1.1.1.37 0.0098
-
NADH 30°C Thermus thermophilus
1.1.1.37 0.019
-
L-malate 30°C Thermus thermophilus
1.1.1.37 0.02
-
oxaloacetate 60°C Thermus thermophilus
1.1.1.37 0.026
-
NAD+ 30°C Thermus thermophilus
1.1.1.37 0.03
-
NADH 60°C Thermus thermophilus
1.1.1.37 0.095
-
L-malate 60°C Thermus thermophilus
1.1.1.37 0.14
-
NAD+ 60°C Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 mitochondrion
-
Thermus thermophilus 5739
-
1.1.1.37 mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 35000
-
2 * 35000, SDS-PAGE Thermus thermophilus
1.1.1.37 60000
-
-
Escherichia coli
1.1.1.37 60000
-
-
Sus scrofa
1.1.1.37 60000
-
gel filtration Thermus thermophilus
1.1.1.37 67000
-
-
Sus scrofa
1.1.1.37 67000
-
sedimentation equilibrium centrifugation Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Bacillus subtilis
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Thermus thermophilus
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Sus scrofa
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Geobacillus stearothermophilus
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Comamonas testosteroni
-
oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+ Thermus thermophilus AT-62
-
oxaloacetate + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Bacillus subtilis
-
-
-
1.1.1.37 Comamonas testosteroni
-
-
-
1.1.1.37 Escherichia coli
-
-
-
1.1.1.37 Geobacillus stearothermophilus
-
-
-
1.1.1.37 Sus scrofa
-
pig
-
1.1.1.37 Thermus thermophilus
-
AT-62
-
1.1.1.37 Thermus thermophilus AT-62
-
AT-62
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
Thermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 heart
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Thermus thermophilus oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Sus scrofa oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Comamonas testosteroni oxaloacetate + NADH + H+
-
r
1.1.1.37 (S)-malate + NAD+
-
Thermus thermophilus AT-62 oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Thermus thermophilus oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Sus scrofa oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Comamonas testosteroni oxaloacetate + NADH + H+
-
r
1.1.1.37 malate + NAD+
-
Thermus thermophilus AT-62 oxaloacetate + NADH + H+
-
r
1.1.1.37 oxaloacetate + NADH
-
Bacillus subtilis L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Thermus thermophilus L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Escherichia coli L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Sus scrofa L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Geobacillus stearothermophilus L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Comamonas testosteroni L-malate + NAD+
-
r
1.1.1.37 oxaloacetate + NADH
-
Thermus thermophilus AT-62 L-malate + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer
-
Sus scrofa
1.1.1.37 dimer
-
Comamonas testosteroni
1.1.1.37 dimer 2 * 35000, SDS-PAGE Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 90
-
remarkably heat stable without losing activity after incubation for 60 min, 50% activity lost after 30 min at 96°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.2
-
oxaloacetate reduction, 60°C Thermus thermophilus
1.1.1.37 9.5
-
30°C Thermus thermophilus