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Literature summary extracted from
- Methylobacterium rhodesianum MB 126 possesses two acetoacetyl-CoA reductases (1994), Arch. Microbiol., 161, 277-280.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.36 | acetoacetyl-CoA | NADH-linked, less sensitive; NADPH-linked, concentration above 0.025 mM, nearly independent of NADPH concentrations 0.03-0.5 mM | Methylorubrum rhodesianum |  |
| 1.1.1.36 | acetoacetyl-CoA | - |
Zoogloea ramigera | |
| 1.1.1.36 | additional information | NADH-linked: not inhibited by NADPH at concentrations of 1 mM, NADPH-linked: not inhibited by NAD+ at concentrations of 1 mM | Methylorubrum rhodesianum | |
| 1.1.1.36 | NAD+ | NADH-linked | Methylorubrum rhodesianum | |
| 1.1.1.36 | NADP+ | - |
Methylorubrum rhodesianum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.36 | 0.015 | - |
acetoacetyl-CoA | cofactor NADPH | Methylorubrum rhodesianum | |
| 1.1.1.36 | 0.018 | - |
NADPH | - |
Methylorubrum rhodesianum | |
| 1.1.1.36 | 0.022 | - |
L-beta-hydroxybutyryl-CoA | - |
Methylorubrum rhodesianum | |
| 1.1.1.36 | 0.03 | - |
D-beta-hydroxybutyryl-CoA | - |
Methylorubrum rhodesianum | |
| 1.1.1.36 | 0.03 | - |
acetoacetyl-CoA | cofactor NADH | Methylorubrum rhodesianum | |
| 1.1.1.36 | 0.06 | - |
NADP+ | - |
Methylorubrum rhodesianum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.36 | 42000 | - |
NADH-linked enzyme: x * 55000, NADPH-linked enzyme: x * 42000, SDS-PAGE | Methylorubrum rhodesianum |
| 1.1.1.36 | 250000 | - |
NADPH-linked: gel filtration | Methylorubrum rhodesianum |
| 1.1.1.36 | 340000 | - |
NADH-linked: gel filtration | Methylorubrum rhodesianum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Zoogloea ramigera | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Rhodospirillum rubrum | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Zoogloea ramigera | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Rhodococcus ruber | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.36 | Methylorubrum rhodesianum | - |
MB 126 | - |
| 1.1.1.36 | Methylorubrum rhodesianum MB 126 | - |
MB 126 | - |
| 1.1.1.36 | Rhodococcus ruber | - |
- |
- |
| 1.1.1.36 | Rhodospirillum rubrum | - |
- |
- |
| 1.1.1.36 | Zoogloea ramigera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.36 | NADH-linked: DEAE-Sepharose CL-6B, Blue Sepharose CL-6B, botyl Sepharose 4B, Mono Q 5/5 | Methylorubrum rhodesianum |
| 1.1.1.36 | NADPH-linked: DEAE-Sepharose CL-6B, Red 120-agarose, phenyle Sepharose | Methylorubrum rhodesianum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.36 | 400 | - |
NADPH-linked | Methylorubrum rhodesianum |
| 1.1.1.36 | 771 | - |
NADH-linked, 345 with NADPH | Methylorubrum rhodesianum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | reverse only with L-form | Methylorubrum rhodesianum | L-beta-hydroxybutyryl-CoA + NAD+ | - |
r | |
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | reverse only with L-form | Methylorubrum rhodesianum MB 126 | L-beta-hydroxybutyryl-CoA + NAD+ | - |
r | |
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Zoogloea ramigera | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Rhodospirillum rubrum | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | reverse only with D-form | Methylorubrum rhodesianum | D-beta-hydroxybutyryl-CoA + NADP+ | - |
r | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | reverse only with D-form | Methylorubrum rhodesianum MB 126 | D-beta-hydroxybutyryl-CoA + NADP+ | - |
r | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Zoogloea ramigera | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
| 1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Rhodococcus ruber | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.36 | ? | NADH-linked enzyme: x * 55000, NADPH-linked enzyme: x * 42000, SDS-PAGE | Methylorubrum rhodesianum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.36 | 7 | - |
NADPH-linked, reverse reaction pH 10.0 | Methylorubrum rhodesianum |
| 1.1.1.36 | 7 | - |
NADH-linked, reverse reaction pH 9.5 | Methylorubrum rhodesianum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.36 | NADH | - |
Zoogloea ramigera | |
| 1.1.1.36 | NADH | - |
Rhodospirillum rubrum | |
| 1.1.1.36 | NADH | - |
Methylorubrum rhodesianum | |
| 1.1.1.36 | NADPH | - |
Zoogloea ramigera | |
| 1.1.1.36 | NADPH | - |
Rhodococcus ruber | |
| 1.1.1.36 | NADPH | - |
Methylorubrum rhodesianum | |
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