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Literature summary extracted from

  • Belova, L.L.; Sokolov, A.P.; Sidorov, I.A.; Trotsenko, Y.A.
    Purification and characterization of NADPH-dependent acetoacetyl-CoA reductase from Methylobacterium extorquens (1997), FEMS Microbiol. Lett., 156, 275-279.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.36 alpha-ketoglutarate 20% activation Methylorubrum extorquens
1.1.1.36 ATP 32% activation Methylorubrum extorquens
1.1.1.36 citrate 30% activation Methylorubrum extorquens
1.1.1.36 isocitrate 25% activation, stimulates NADP+ regeneration Methylorubrum extorquens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.36 acetoacetyl-CoA
-
 Azotobacter vinelandii
1.1.1.36 acetoacetyl-CoA
-
 Cupriavidus necator
1.1.1.36 acetoacetyl-CoA concentration above 40 mM Methylorubrum extorquens
1.1.1.36 acetoacetyl-CoA
-
 Methylorubrum rhodesianum
1.1.1.36 acetoacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 NAD+ 17% inhibition Methylorubrum extorquens
1.1.1.36 NAD+
-
 Methylorubrum rhodesianum
1.1.1.36 NADP+ 40% inhibition Methylorubrum extorquens
1.1.1.36 NADP+
-
 Methylorubrum rhodesianum
1.1.1.36 NADPH
-
 Azotobacter vinelandii
1.1.1.36 NADPH concentrationgreater 100 mM Methylorubrum extorquens
1.1.1.36 NADPH
-
 Methylorubrum rhodesianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.36 0.005
-
 acetoacetyl-CoA
-
 Cupriavidus necator
1.1.1.36 0.011
-
 acetoacetyl-CoA 100 mM NADPH Azotobacter vinelandii
1.1.1.36 0.0116
-
 acetoacetyl-CoA
-
 Methylorubrum extorquens
1.1.1.36 0.015
-
 acetoacetyl-CoA
-
 Methylorubrum rhodesianum
1.1.1.36 0.018
-
 NADPH
-
 Methylorubrum rhodesianum
1.1.1.36 0.019
-
 NADPH
-
 Cupriavidus necator
1.1.1.36 0.02
-
 NADPH 32 mM acetoacetyl-CoA Azotobacter vinelandii
1.1.1.36 0.03
-
 D-beta-hydroxybutyryl-CoA
-
 Methylorubrum rhodesianum
1.1.1.36 0.031
-
 NADP+
-
 Cupriavidus necator
1.1.1.36 0.033
-
 D-hydroxybutyryl-CoA
-
 Cupriavidus necator
1.1.1.36 0.041
-
 NADPH
-
 Methylorubrum extorquens
1.1.1.36 0.06
-
 NADP+
-
 Methylorubrum rhodesianum
1.1.1.36 0.25
-
 NAD+
-
 Cupriavidus necator
1.1.1.36 0.4
-
 NADH
-
 Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.36 soluble
-
 Zoogloea ramigera
-
-
1.1.1.36 soluble
-
 Methylorubrum extorquens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.36 31000
-
 4 * 31000, 12% PAGE Methylorubrum extorquens
1.1.1.36 77000
-
-
 Azotobacter vinelandii
1.1.1.36 84000
-
-
 Cupriavidus necator
1.1.1.36 92000
-
-
 Zoogloea ramigera
1.1.1.36 140000
-
 gel-filtration Methylorubrum extorquens
1.1.1.36 141000
-
 gradient PAGE Methylorubrum extorquens
1.1.1.36 250000
-
-
 Methylorubrum rhodesianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+ Zoogloea ramigera
-
 L-3-hydroxybutyryl-CoA + NAD+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADH + H+ Rhodospirillum rubrum
-
 L-3-hydroxybutyryl-CoA + NAD+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Zoogloea ramigera PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Cupriavidus necator PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Methylorubrum extorquens tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Methylorubrum extorquens 15 tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway D-3-hydroxybutyryl-CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Azotobacter vinelandii
-
-
-
1.1.1.36 Cupriavidus necator
-
-
-
1.1.1.36 Methylorubrum extorquens
-
 15
-
1.1.1.36 Methylorubrum extorquens 15
-
 15
-
1.1.1.36 Methylorubrum rhodesianum
-
 MB 126
-
1.1.1.36 Methylorubrum rhodesianum MB 126
-
 MB 126
-
1.1.1.36 Rhodospirillum rubrum
-
-
-
1.1.1.36 Zoogloea ramigera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 PEG, DEAE-Toyopearl column Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 184
-
-
 Methylorubrum extorquens

Storage Stability

EC Number Storage Stability Organism
1.1.1.36 0°C, 25 mM Tris-HCl, pH 8.0 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+
-
 Zoogloea ramigera L-3-hydroxybutyryl-CoA + NAD+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADH + H+
-
 Cupriavidus necator L-3-hydroxybutyryl-CoA + NAD+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADH + H+
-
 Rhodospirillum rubrum L-3-hydroxybutyryl-CoA + NAD+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Methylorubrum rhodesianum D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Methylorubrum rhodesianum MB 126 D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Zoogloea ramigera D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Cupriavidus necator D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway Methylorubrum extorquens D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway Methylorubrum extorquens 15 D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Azotobacter vinelandii (R)-3-hydroxybutanoyl-CoA +NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.36 tetramer
-
 Zoogloea ramigera
1.1.1.36 tetramer 4 * 31000, 12% PAGE Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.36 30
-
-
 Zoogloea ramigera
1.1.1.36 30
-
-
 Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.36 7.5
-
-
 Methylorubrum extorquens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADPH
-
 Azotobacter vinelandii
1.1.1.36 NADPH
-
 Cupriavidus necator
1.1.1.36 NADPH
-
 Methylorubrum rhodesianum
1.1.1.36 NADPH absolute specificity Zoogloea ramigera
1.1.1.36 NADPH absolute specificity Methylorubrum extorquens