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Literature summary extracted from

  • Saito, T.; Fukui, T.; Ikeda, F.; Tanaka, Y.; Tomita, K.
    An NADP-linked acetoacetyl CoA reductase from Zoogloea ramigera (1977), Arch. Microbiol., 114, 211-217.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.36 acetoacetyl-CoA above 0.01 mM Zoogloea ramigera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.36 0.0083
-
 acetoacetyl-CoA cofactor NADPH Zoogloea ramigera
1.1.1.36 0.02 0.021 NADPH cosubstrate acetoacetyl-CoA Zoogloea ramigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+ Zoogloea ramigera low activity L-3-hydroxybutyryl-CoA + NAD+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Zoogloea ramigera high activity D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Zoogloea ramigera PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Azotobacter beijerinckii PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Azotobacter beijerinckii
-
-
-
1.1.1.36 Zoogloea ramigera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 ammonium sulfate fractionation, hydroxylapatite, Sephadex G-200 chromatography Zoogloea ramigera

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 7.7
-
 NADPH-linked Zoogloea ramigera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+ low activity Zoogloea ramigera L-3-hydroxybutyryl-CoA + NAD+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Zoogloea ramigera (3R)-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Azotobacter beijerinckii (3R)-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ high activity Zoogloea ramigera D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Zoogloea ramigera D-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Azotobacter beijerinckii D-3-hydroxybutyryl-CoA + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.36 25
-
-
 Zoogloea ramigera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.36 8.1
-
 reduction of acetoacetyl-CoA Zoogloea ramigera

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADH
-
 Zoogloea ramigera
1.1.1.36 NADH
-
 Azotobacter beijerinckii
1.1.1.36 NADP+
-
 Zoogloea ramigera
1.1.1.36 NADP+
-
 Azotobacter beijerinckii
1.1.1.36 NADPH
-
 Zoogloea ramigera
1.1.1.36 NADPH
-
 Azotobacter beijerinckii