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Literature summary extracted from

  • He, X.Y.; Yang, S.Y.
    Molecular cloning, expression in Escherichia coli, and characterization of a novel L-3-hydroxyacyl coenzyme A dehydrogenase from pig liver (1998), Biochim. Biophys. Acta, 1392, 119-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 expressed in Escherichia coli Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 0.003
-
 NADH
-
 Sus scrofa
1.1.1.35 0.003
-
 acetoacetyl-CoA
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.35 mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 33000
-
 2 * 33000, SDS-PAGE Sus scrofa
1.1.1.35 66000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Sus scrofa
-
 acetoacetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 overexpressed in Escherichia coli, phosphocellulose Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.35 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 511
-
 recombinant enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Sus scrofa acetoacetyl-CoA + NADH
-
 r
1.1.1.35 3-ketohexadecanoyl-CoA + NADH
-
 Sus scrofa (S)-3-hydroxhexadecanoyl-CoA + NAD+
-
 r
1.1.1.35 3-ketooctanoyl-CoA + NADH
-
 Sus scrofa (S)-3-hydroxyoctanoyl-CoA + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.35 dimer 2 * 33000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.35 45
-
 3-ketohexadecanoyl-CoA
-
 Sus scrofa
1.1.1.35 102
-
 3-ketooctanoyl-CoA
-
 Sus scrofa
1.1.1.35 330
-
 acetoacetyl-CoA pH 7.0 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Sus scrofa