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Literature summary extracted from

  • Montalvetti, A.; Pena-Diaz, J.; Hurtado, R.; Ruiz-Perez, L.M.; Gonzales-Pacanowska, D.
    Characterization and regulation of Leishmania major 3-hydroxy-3-methylglutaryl-CoA reductase (2000), Biochem. J., 349, 27-34.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 overexpression in Escherichia coli Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.34 0.04
-
 (R,S)-3-hydroxy-3-methylglutaryl-CoA
-
 Leishmania major
1.1.1.34 0.081
-
 NADPH
-
 Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.34 46000
-
 alpha4, 4 * 46000, SDS-PAGE Leishmania major
1.1.1.34 184000
-
 gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Leishmania major
-
 (R)-mevalonate + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34
-
 Leishmania major

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 0.0335
-
-
 Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Leishmania major (R)-mevalonate + CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.34 tetramer alpha4, 4 * 46000, SDS-PAGE Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
 Leishmania major