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Literature summary extracted from

  • Thompson, E.D.; Nakata, H.M.
    Characterization and partial purification of beta-hydroxybutyrate dehydrogenase from sporulating cells of Bacillus cereus T (1973), Can. J. Microbiol., 19, 673-677.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.95
-
 NAD+
-
 Bacillus cereus
1.1.1.30 2.3
-
 (R)-3-hydroxybutanoate
-
 Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.30 Mg2+ 0.1 mM, 2fold increase in activity, crude extracts Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Bacillus cereus
-
 acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Bacillus cereus T
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Bacillus cereus
-
-
-
1.1.1.30 Bacillus cereus T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 ammonium sulfate precipitation, DEAE-Sephadex, Sephadex G-200 Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 21
-
-
 Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
1.1.1.30 4°C, Tris-HCl buffer, 50-100 mM Mg2+, Mn2+, Ba2+, or Ca2+, enzyme loses all activity in the presence of phosphate buffer or EDTA within 2 min Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bacillus cereus acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bacillus cereus T acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bacillus cereus acetoacetate + NADH + H+
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bacillus cereus T acetoacetate + NADH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 8
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.30 7 9
-
 Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Bacillus cereus