Literature summary extracted from

Phelps, D.C.; Hatefi, Y.
Inhibition of D(-)-beta-hydroxybutyrate dehydrogenase by butanedione, phenylglyoxal, and diethyl pyrocarbonate (1981), Biochemistry, 20, 459-463.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.30	phosphatidylcholine	-	Bos taurus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.30	Butanedione	-	Bos taurus
1.1.1.30	diazenedicarboxylic acid bis(dimethylamide)	0.20 mM, activity can be recovered with dithiothreitol, enzyme contains a vicinal dithiol that is oxidized by diamide	Bos taurus
1.1.1.30	N-ethylmaleimide	irreversible inhibition	Bos taurus
1.1.1.30	Phenylglyoxal	extremely rapid inhibition	Bos taurus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.30	0.28	-	3-acetylpyridine adenine dinucleotide	-	Bos taurus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.30	mitochondrion	-	Bos taurus	5739	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.30	(R)-3-hydroxybutanoate + NAD+	Bos taurus	-	acetoacetate + NADH	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.30	Bos taurus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.30	heart	-	Bos taurus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.30	0.02	-	cofactor 3-acetylpyridine adenine dinucleotide	Bos taurus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.30	(R)-3-hydroxybutanoate + 3-acetylpyridine adenine dinucleotide	-	Bos taurus	acetoacetate + 3-acetylpyridine adenine dinucleotideH2	-	r
1.1.1.30	(R)-3-hydroxybutanoate + NAD+	-	Bos taurus	acetoacetate + NADH	-	r

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.30	NAD+	-	Bos taurus

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Release 2023.1 (January 2023)