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Literature summary extracted from

  • Burnett, B.K.; Khorana, H.G.
    A rapid and efficient procedure for the purification of mitochondrial beta-hydroxybutyrate dehydrogenase (1985), Biochim. Biophys. Acta, 815, 51-56.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.30 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 31500
-
 alpha4, 4 * 31500, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Bos taurus
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 ammonium sulfate, acid precipitation, DEAE-Sepharose, glass beads Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 100
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 tetramer alpha4, 4 * 31500, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Bos taurus