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Literature summary extracted from

  • Matyskova I.; Kovar, J.; Racek, P.
    Purification and properties of D-3-hydroxybutyrate dehydrogenase from Paracoccus denitrificans (1985), Biochim. Biophys. Acta, 839, 300-307.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 32000
-
 alpha4, 4 * 32000, SDS-PAGE Paracoccus denitrificans
1.1.1.30 132000
-
 gel filtration Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Paracoccus denitrificans
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Paracoccus denitrificans
-
 enzyme shows hysteresis, lag phase in progress curve
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 97
-
-
 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
1.1.1.30 -20°C, several months, 0%, 4°C, lyophilized, 2 month, 0% inactivation, 4°C, 0.1 M sodium phosphate buffer pH 7-8, 5 days, 50% inactivation, stability increases in the presence of 1-10 mM EDTA Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Paracoccus denitrificans acetoacetate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 tetramer alpha4, 4 * 32000, SDS-PAGE Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Paracoccus denitrificans