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Literature summary extracted from

  • LeJohn, H.B.; Stevenson, R.M.
    D(-)-lactate dehydrogenase from fungi (1975), Methods Enzymol., 41B, 293-298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.28 fructose 1,6-diphosphate
-
 Globisporangium debaryanum
1.1.1.28 fructose 1,6-diphosphate
-
 Sapromyces elongatus
1.1.1.28 fructose 1,6-diphosphate
-
 Elongisporangium undulatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 98000
-
 sucrose density gradient sedimentation Globisporangium debaryanum
1.1.1.28 115000
-
 gel filtration Globisporangium debaryanum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Globisporangium debaryanum
-
 pyruvate + NADH
-
 r
1.1.1.28 (R)-lactate + NAD+ Sapromyces elongatus
-
 pyruvate + NADH
-
 r
1.1.1.28 (R)-lactate + NAD+ Elongisporangium undulatum
-
 pyruvate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Elongisporangium undulatum
-
-
-
1.1.1.28 Globisporangium debaryanum
-
-
-
1.1.1.28 Sapromyces elongatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Globisporangium debaryanum
1.1.1.28
-
 Sapromyces elongatus
1.1.1.28
-
 Elongisporangium undulatum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ binary-binary mechanism with either NAD+ or NADH as the first substrate Globisporangium debaryanum
a
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ binary-binary mechanism with either NAD+ or NADH as the first substrate Sapromyces elongatus
a
1.1.1.28 (R)-lactate + NAD+ = pyruvate + NADH + H+ binary-binary mechanism with either NAD+ or NADH as the first substrate Elongisporangium undulatum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.28 mycelium
-
 Globisporangium debaryanum
-
1.1.1.28 mycelium
-
 Sapromyces elongatus
-
1.1.1.28 mycelium
-
 Elongisporangium undulatum
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.28 -20°C, for at least 2 years Globisporangium debaryanum
1.1.1.28 -20°C, for at least 2 years Sapromyces elongatus
1.1.1.28 -20°C, for at least 2 years Elongisporangium undulatum
1.1.1.28 4°C, several weeks Globisporangium debaryanum
1.1.1.28 4°C, several weeks Sapromyces elongatus
1.1.1.28 4°C, several weeks Elongisporangium undulatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Globisporangium debaryanum pyruvate + NADH
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Sapromyces elongatus pyruvate + NADH
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Elongisporangium undulatum pyruvate + NADH
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Globisporangium debaryanum
1.1.1.28 NAD+
-
 Sapromyces elongatus
1.1.1.28 NAD+
-
 Elongisporangium undulatum
1.1.1.28 NADH
-
 Globisporangium debaryanum
1.1.1.28 NADH
-
 Sapromyces elongatus
1.1.1.28 NADH
-
 Elongisporangium undulatum