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Literature summary extracted from

  • Savijoki, K.; Palva, A.
    Molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and biochemical characterization of the enzyme (1997), Appl. Environ. Microbiol., 63, 2850-2856.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27
-
 Lactobacillus helveticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 Cd2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
1.1.1.27 Co2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
1.1.1.27 Cu2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
1.1.1.27 HEPES buffer
-
 Lactobacillus helveticus
1.1.1.27 MES buffer
-
 Lactobacillus helveticus
1.1.1.27 p-hydroxymercuribenzoate
-
 Lactobacillus helveticus
1.1.1.27 Tris-HCl
-
 Lactobacillus helveticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.25
-
 pyruvate
-
 Lactobacillus helveticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 Ca2+ 1 mM, almost 2fold stimulation Lactobacillus helveticus
1.1.1.27 Zn2+ 1 mM, almost 2fold stimulation Lactobacillus helveticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 125000 138000 gel filtration Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lactobacillus helveticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 2119
-
 recombinant enzyme Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 2-oxobutyrate + NADH 3% of the activity with pyruvate Lactobacillus helveticus 2-hydroxybutyrate + NAD+
-
 ?
1.1.1.27 phenylpyruvate + NADH 28% of the activity with pyruvate Lactobacillus helveticus 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Lactobacillus helveticus (S)-lactate + NAD+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 50
-
 10 min, 90% loss of activity Lactobacillus helveticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.27 643
-
 pyruvate
-
 Lactobacillus helveticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 5
-
-
 Lactobacillus helveticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NADH coenzyme Lactobacillus helveticus