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Literature summary extracted from

  • Crow, V.L.; Pritchard, G.G.
    Fructose 1,6-diphosphate-activated L-lactate dehydrogenase from Streptococcus lactis: kinetic properties and factors affecting activation (1977), J. Bacteriol., 131, 82-91.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 phosphate
-
 Lactococcus lactis
1.1.1.27 pyruvate above 20 mM Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.08
-
 NADH pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate Lactococcus lactis
1.1.1.27 1.5
-
 pyruvate pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate Lactococcus lactis
1.1.1.27 2.4
-
 NAD+ in presence of 1 mM fructose 1,6-diphosphate Lactococcus lactis
1.1.1.27 8
-
 NADH without fructose 1,6-diphosphate Lactococcus lactis
1.1.1.27 100
-
 (S)-lactate in presence of 1 mM fructose 1,6-diphosphate Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 Mn2+ no effect Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 additional information Lactococcus lactis the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 109
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ inactive towards D-lactate Lactococcus lactis pyruvate + NADH + H+
-
 r
1.1.1.27 additional information the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions Lactococcus lactis ?
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Lactococcus lactis (S)-lactate + NAD+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6.9
-
 in presence of fructose 1,6-diphosphate Lactococcus lactis
1.1.1.27 8.2
-
 in absence of fructose 1,6-diphosphate Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Lactococcus lactis
1.1.1.27 NADH coenzyme Lactococcus lactis