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Literature summary extracted from

  • Altincicek, B.; Hintz, M.; Sanderbrand, S.; Wiesner, J.; Beck, E.; Jomaa, H.
    Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa (2000), FEMS Microbiol. Lett., 190, 329-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 overexpression in Escherichia coli Pseudomonas aeruginosa
2.2.1.7 expressed in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 fosmidomycin
-
 Pseudomonas aeruginosa
1.1.1.267 FR-900098
-
 Pseudomonas aeruginosa
2.2.1.7 Fluoropyruvate recombinant protein, 50% inhibition at 0.4 mM, fluoropyruvate is supposed to bind covalently to the active site Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Pseudomonas aeruginosa
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Pseudomonas aeruginosa
-
-
-
2.2.1.7 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 of recombinant protein Pseudomonas aeruginosa
2.2.1.7 histidine-tagged recombinant protein, expressed in Escherichia coli Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Pseudomonas aeruginosa 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ir
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Pseudomonas aeruginosa 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?
2.2.1.7 pyruvate + DL-glyceraldehyde 3-phosphate
-
 Pseudomonas aeruginosa 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 DXP-reductoisomerase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
-
 Pseudomonas aeruginosa