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Literature summary extracted from

  • Lourenco, E.J.; Silva, G.M.L.; Neves, V.A.
    Purification and properties of shikimate dehydrogenase from cucumber (Cucumis sativus L.). (1991), J. Agric. Food Chem., 39, 458-462.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.25 stable in crude extract Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 Cd2+ 500 nM Cucumis sativus
1.1.1.25 HgCl2 complete inhibition at concentration 0.05 mM Cucumis sativus
1.1.1.25 p-chloromercuribenzoate biphasic inhibition: first rapid inhibition leading to loss of 70% activity, then within 5 min loss of the remaining 30% activity, inhibition can be partially hindered by thiols and chloride Cucumis sativus
1.1.1.25 protocatechuic acid
-
 Cucumis sativus
1.1.1.25 Zn2+ 0.005 mM Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.01
-
 NADP+
-
 Cucumis sativus
1.1.1.25 0.05
-
 shikimic acid NADP+ Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 45000
-
 gel filtration, 2 different isoenzymes with molecular weight about 45000 Da Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Cucumis sativus
-
 3-dehydroshikimate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Cucumis sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25
-
 Cucumis sativus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 3.5
-
 crude extract Cucumis sativus
1.1.1.25 25.4
-
 purified enzyme Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
 Cucumis sativus 3-dehydroshikimate + NADPH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 50
-
-
 Cucumis sativus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.25 20 55
-
 Cucumis sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.25 60
-
 complete inactivation after 10 min, at 50-55°C biphasic, inactivation can be minimized with bound substrate to about 60% Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 8.7
-
-
 Cucumis sativus