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Literature summary extracted from

  • Trower, M.K.; Buckland, R.M.; Higgins, R.; Griffin, M.
    Isolation and characterization of a cyclohexane-metabolizing Xanthobacter sp. (1985), Appl. Environ. Microbiol., 49, 1282-1289.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.245 0.0013
-
 Cyclohexanol pH 10.3 Xanthobacter sp.
1.1.1.245 0.41
-
 Cyclohexane-1,2-diol pH 10.3 Xanthobacter sp.
1.1.1.245 0.5
-
 Cyclohexane-1,3-diol pH 10.3 Xanthobacter sp.
1.1.1.245 0.59
-
 Cyclohexane-1,4-diol pH 10.3 Xanthobacter sp.
1.14.13.22 0.0005
-
 cyclohexanone below Xanthobacter sp.
1.14.13.22 0.0093
-
 2-Hydroxycyclohexanone
-
 Xanthobacter sp.
1.14.13.22 0.143
-
 Cyclohexan-1,2-dione
-
 Xanthobacter sp.
1.14.13.22 0.19
-
 Cyclohexan-1,4-dione
-
 Xanthobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.22 cytoplasm
-
 Xanthobacter sp. 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.245 cyclohexanol + NAD+ Xanthobacter sp. metabolism of cyclohexane cyclohexanone + NADH
-
 r
1.14.13.22 1-oxa-2-oxocycloheptane + NADPH + O2 Xanthobacter sp. oxidation ?
-
 ?
1.14.13.22 cyclohexan-1,2-dione + NADPH + O2 Xanthobacter sp.
-
 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexan-1,4-dione + NADPH + O2 Xanthobacter sp.
-
 1-oxa-2,5-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 Xanthobacter sp.
-
 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.245 Xanthobacter sp.
-
-
-
1.14.13.22 Xanthobacter sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.245 0.87
-
-
 Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.245 cyclohexan-1,2-diol + NAD+
-
 Xanthobacter sp. cyclohexane-1,2-dione + NADH
-
 ?
1.1.1.245 cyclohexane-1,3-diol + NAD+
-
 Xanthobacter sp. cyclohexane-1,3-dione + NADH
-
 ?
1.1.1.245 cyclohexane-1,4-diol + NAD+
-
 Xanthobacter sp. cyclohexane-1,4-dione + NADH
-
 ?
1.1.1.245 cyclohexanol + NAD+ preferred substrate Xanthobacter sp. cyclohexanone + NADH
-
 ?
1.1.1.245 cyclohexanol + NAD+ metabolism of cyclohexane Xanthobacter sp. cyclohexanone + NADH
-
 r
1.1.1.245 additional information cyclohexane-1,2-dione is not oxidized with NAD+ Xanthobacter sp. additional information
-
 ?
1.14.13.22 1-oxa-2-oxocycloheptane + NADPH + O2 oxidation Xanthobacter sp. ?
-
 ?
1.14.13.22 2-hydroxycyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexan-1,2-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexan-1,4-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,5-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 6-hexanolide + NADP+ + H2O product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 additional information absolutely specific for NADPH as electron donor Xanthobacter sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.245 10.1 10.5 oxidation of alcohol Xanthobacter sp.
1.14.13.22 6.5
-
-
 Xanthobacter sp.
1.14.13.22 8.5 9
-
 Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.245 NAD+
-
 Xanthobacter sp.
1.1.1.245 NADP+ weaker activity than with NAD+ Xanthobacter sp.
1.14.13.22 NADH no activity Xanthobacter sp.
1.14.13.22 NADPH absolutely specific for NADPH as electron donor Xanthobacter sp.