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Literature summary extracted from

  • Stich, K.; Forkmann, G.
    Biosynthesis of 3-deoxyanthocyanins with flower extracts from Sinningia cardinalis (1988), Biochemistry, 27, 785-789.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.234 freezing fresh flowers in liquid nitrogen and storing them at -80°C enhances extractable activity compared to fresh flowers Sinningia cardinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.234 glycerol 10% v/v, complete irreversible inactivation Sinningia cardinalis
1.1.1.234 additional information no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each Sinningia cardinalis
1.1.1.234 naringenin above 0.1 mM Sinningia cardinalis
1.1.1.234 p-chloromercuribenzoate 0.5 mM Sinningia cardinalis
1.1.1.234 sucrose 20% w/v, complete irreversible inactivation Sinningia cardinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.234 0.021 0.023 (2S)-naringenin
-
 Sinningia cardinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.234 (2S)-flavanone + NADPH Sinningia cardinalis involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol (2S)-flavan-4-ol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.234 Sinningia cardinalis
-
 syn. Rechsteineria cardinalis, strain " Feuerschein"
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.234 flower
-
 Sinningia cardinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.234 0.015
-
-
 Sinningia cardinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.234 (2S)-eriodictyol + NADPH
-
 Sinningia cardinalis luteoforol + NADP+
-
 ?
1.1.1.234 (2S)-flavanone + NADPH involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol Sinningia cardinalis (2S)-flavan-4-ol + NADP+
-
 ?
1.1.1.234 (2S)-naringenin + NADPH + H+
-
 Sinningia cardinalis apiforol + NADP+
-
 ?
1.1.1.234 5,7,3',4',5'-pentahydroxyflavanone + NADPH
-
 Sinningia cardinalis 5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+
-
 ?
1.1.1.234 additional information as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase Sinningia cardinalis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.234 6
-
-
 Sinningia cardinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.234 4.7 6.9 about half-maximal activity at pH 4.7 and 6.9 Sinningia cardinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.234 additional information no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH Sinningia cardinalis
1.1.1.234 NADH 77% as active as NADPH Sinningia cardinalis
1.1.1.234 NADPH required Sinningia cardinalis