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Literature summary extracted from

  • Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.
    Purification and properties of an asymmetric reduction enzyme of 2-methyl-3-oxobutyrate in baker's yeast (1985), Agric. Biol. Chem., 49, 2563-2570.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.217 FMN
-
 Saccharomyces cerevisiae
1.1.1.217 KCN
-
 Saccharomyces cerevisiae
1.1.1.217 NADH
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.217 0.42
-
 Benzyl 2-methyl-3-oxobutyrate
-
 Saccharomyces cerevisiae
1.1.1.217 4.2
-
 benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
-
 Saccharomyces cerevisiae
1.1.1.217 4.2
-
 benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.217 Ag+ strong inhibition Saccharomyces cerevisiae
1.1.1.217 Cu2+ strong inhibition Saccharomyces cerevisiae
1.1.1.217 Hg2+ strong inhibition Saccharomyces cerevisiae
1.1.1.217 Ni2+ strong inhibition Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.217 31000
-
 SDS-PAGE Saccharomyces cerevisiae
1.1.1.217 32000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.217 2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+ Saccharomyces cerevisiae
-
 benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.217 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.217
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.217 0.0063
-
 crude extract Saccharomyces cerevisiae
1.1.1.217 0.9
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.217 2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
 Saccharomyces cerevisiae benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
 r
1.1.1.217 2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
 Saccharomyces cerevisiae benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+ ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme r
1.1.1.217 additional information no reduction of aldehydes or alcohols Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.217 monomer
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.217 45
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.217 30 60
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.217 40
-
 rapid loss of activity above 40°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.217 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.217 5 8 less than 10% activity at pH 4 and 9 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.217 6 8 purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.217 NADP+
-
 Saccharomyces cerevisiae