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Literature summary extracted from

  • Ameyama, M.; Adachi, O.
    2-keto-D-Gluconate reductase from acetic acid bacteria (1982), Methods Enzymol., 89, 203-210.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.215
-
 Acetobacter pasteurianus
1.1.1.215
-
 Gluconacetobacter liquefaciens
1.1.1.215
-
 Acetobacter ascendens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.215 Hg2+ complete inhibition Acetobacter ascendens
1.1.1.215 Hg2+ complete inhibition Acetobacter pasteurianus
1.1.1.215 Hg2+ complete inhibition Gluconacetobacter liquefaciens
1.1.1.215 Hg2+ complete inhibition Gluconobacter oxydans
1.1.1.215 sulfhydryl reagents strong Acetobacter ascendens
1.1.1.215 sulfhydryl reagents strong Acetobacter pasteurianus
1.1.1.215 sulfhydryl reagents strong Gluconacetobacter liquefaciens
1.1.1.215 sulfhydryl reagents strong Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 cytoplasm
-
 Gluconobacter oxydans 5737
-
1.1.1.215 cytoplasm
-
 Acetobacter pasteurianus 5737
-
1.1.1.215 cytoplasm
-
 Gluconacetobacter liquefaciens 5737
-
1.1.1.215 cytoplasm
-
 Acetobacter ascendens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.215 additional information no metal requirement Gluconobacter oxydans
1.1.1.215 additional information no metal requirement Acetobacter pasteurianus
1.1.1.215 additional information no metal requirement Gluconacetobacter liquefaciens
1.1.1.215 additional information no metal requirement Acetobacter ascendens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 34000
-
 2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens
1.1.1.215 43000
-
 2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens
1.1.1.215 120000
-
 gel filtration Acetobacter pasteurianus
1.1.1.215 120000
-
 gel filtration Gluconacetobacter liquefaciens
1.1.1.215 120000
-
 gel filtration Acetobacter ascendens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 2-dehydro-D-gluconate + NADPH Acetobacter pasteurianus
-
 D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH Gluconobacter oxydans reverse reaction at the same rate by Gluconobacter enzyme D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH Gluconacetobacter liquefaciens reverse reaction at the same rate by Gluconobacter enzyme D-gluconate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Acetobacter ascendens
-
-
-
1.1.1.215 Acetobacter pasteurianus
-
-
-
1.1.1.215 Gluconacetobacter liquefaciens
-
-
-
1.1.1.215 Gluconobacter oxydans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215
-
 Gluconacetobacter liquefaciens
1.1.1.215 affinity chromatography on Blue-Dextran Sepharose B Acetobacter ascendens
1.1.1.215 partial Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 61.03
-
-
 Gluconacetobacter liquefaciens
1.1.1.215 187
-
-
 Acetobacter ascendens
1.1.1.215 336
-
-
 Acetobacter pasteurianus

Storage Stability

EC Number Storage Stability Organism
1.1.1.215 5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Gluconobacter oxydans
1.1.1.215 5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Acetobacter pasteurianus
1.1.1.215 5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Gluconacetobacter liquefaciens
1.1.1.215 5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Acetobacter ascendens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2-dehydro-D-gluconate + NADPH
-
 Acetobacter pasteurianus D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Gluconobacter oxydans D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Gluconacetobacter liquefaciens D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Acetobacter ascendens D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH strongly preferred reaction of Acetobacter enzyme Acetobacter pasteurianus D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH strongly preferred reaction of Acetobacter enzyme Acetobacter ascendens D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH reverse reaction at the same rate by Gluconobacter enzyme Gluconobacter oxydans D-gluconate + NADP+
-
 r
1.1.1.215 2-dehydro-D-gluconate + NADPH reverse reaction at the same rate by Gluconobacter enzyme Gluconacetobacter liquefaciens D-gluconate + NADP+
-
 r
1.1.1.215 2-keto-L-gulonate + NADPH third best substrate Gluconobacter oxydans L-idonate + NADP+
-
 r
1.1.1.215 2-keto-L-gulonate + NADPH third best substrate Gluconacetobacter liquefaciens L-idonate + NADP+
-
 r
1.1.1.215 2-keto-L-gulonate + NADPH third best substrate Acetobacter ascendens L-idonate + NADP+
-
 r
1.1.1.215 2-keto-L-gulonate + NADPH betst substrate Acetobacter pasteurianus L-idonate + NADP+
-
 r
1.1.1.215 5-keto-D-gluconate + NADPH
-
 Gluconobacter oxydans D-gluconate + NADP+
-
 ?
1.1.1.215 5-keto-D-gluconate + NADPH
-
 Gluconacetobacter liquefaciens D-gluconate + NADP+
-
 ?
1.1.1.215 5-keto-D-gluconate + NADPH poor substrate Acetobacter pasteurianus D-gluconate + NADP+
-
 ?
1.1.1.215 5-keto-D-gluconate + NADPH poor substrate Acetobacter ascendens D-gluconate + NADP+
-
 ?
1.1.1.215 D-galactonate + NADP+
-
 Acetobacter pasteurianus 2-dehydro-D-galactonate + NADPH
-
 r
1.1.1.215 D-galactonate + NADP+
-
 Acetobacter ascendens 2-dehydro-D-galactonate + NADPH
-
 r
1.1.1.215 D-galactonate + NADP+ third best substrate Gluconobacter oxydans 2-dehydro-D-galactonate + NADPH
-
 r
1.1.1.215 D-galactonate + NADP+ third best substrate Gluconacetobacter liquefaciens 2-dehydro-D-galactonate + NADPH
-
 r
1.1.1.215 D-xylonate + NADPH
-
 Gluconobacter oxydans 2-keto-D-xylonate + NADP+
-
 ?
1.1.1.215 D-xylonate + NADPH
-
 Acetobacter pasteurianus 2-keto-D-xylonate + NADP+
-
 ?
1.1.1.215 D-xylonate + NADPH
-
 Gluconacetobacter liquefaciens 2-keto-D-xylonate + NADP+
-
 ?
1.1.1.215 L-idonate + NADP+ poor substrate Acetobacter pasteurianus 2-keto-L-idonate + NADPH
-
 ?
1.1.1.215 L-idonate + NADP+ poor substrate Acetobacter ascendens 2-keto-L-idonate + NADPH
-
 ?
1.1.1.215 L-idonate + NADP+ best substrate Gluconobacter oxydans 2-keto-L-idonate + NADPH
-
 ?
1.1.1.215 L-idonate + NADP+ best substrate Gluconacetobacter liquefaciens 2-keto-L-idonate + NADPH
-
 ?
1.1.1.215 additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Gluconobacter oxydans ?
-
 ?
1.1.1.215 additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Acetobacter pasteurianus ?
-
 ?
1.1.1.215 additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Gluconacetobacter liquefaciens ?
-
 ?
1.1.1.215 additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Acetobacter ascendens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 trimer
-
 Acetobacter ascendens
1.1.1.215 trimer 2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.215 55
-
 stable for 5 min, enhanced by the addition of substrate Gluconobacter oxydans
1.1.1.215 55
-
 stable for 5 min, enhanced by the addition of substrate Acetobacter pasteurianus
1.1.1.215 55
-
 stable for 5 min, enhanced by the addition of substrate Gluconacetobacter liquefaciens
1.1.1.215 55
-
 stable for 5 min, enhanced by the addition of substrate Acetobacter ascendens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 6
-
 reduction of 2-ketogluconate Gluconobacter oxydans
1.1.1.215 6
-
 reduction of 2-ketogluconate Acetobacter pasteurianus
1.1.1.215 6
-
 reduction of 2-ketogluconate Gluconacetobacter liquefaciens
1.1.1.215 6
-
 reduction of 2-ketogluconate Acetobacter ascendens
1.1.1.215 10.5
-
-
 Acetobacter ascendens
1.1.1.215 10.5
-
 oxidation of gluconate Gluconacetobacter liquefaciens
1.1.1.215 11
-
 oxidation of gluconate Acetobacter ascendens
1.1.1.215 12
-
 oxidation of gluconate Acetobacter pasteurianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.215 6
-
 crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C Gluconobacter oxydans
1.1.1.215 6
-
 crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C Acetobacter pasteurianus
1.1.1.215 6
-
 crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C Gluconacetobacter liquefaciens
1.1.1.215 6
-
 crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C Acetobacter ascendens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 additional information no activity with NADH Gluconobacter oxydans
1.1.1.215 additional information no activity with NADH Acetobacter pasteurianus
1.1.1.215 additional information no activity with NADH Gluconacetobacter liquefaciens
1.1.1.215 additional information no activity with NADH Acetobacter ascendens
1.1.1.215 NADP+
-
 Gluconobacter oxydans
1.1.1.215 NADP+
-
 Acetobacter pasteurianus
1.1.1.215 NADP+
-
 Gluconacetobacter liquefaciens
1.1.1.215 NADP+
-
 Acetobacter ascendens
1.1.1.215 NADPH
-
 Gluconobacter oxydans
1.1.1.215 NADPH
-
 Acetobacter pasteurianus
1.1.1.215 NADPH
-
 Gluconacetobacter liquefaciens
1.1.1.215 NADPH
-
 Acetobacter ascendens