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Literature summary extracted from
- Fetal lamb 3beta,20alpha-hydroxysteroid oxidoreductase: dual activity at the same active site examined by affinity labeling with 16alpha-(bromo[2-14C]acetoxy)progesterone (1989), Biochemistry, 28, 8856-8863.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.210 | additional information | - |
additional information | kinetic studies | Ovis aries |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.210 | soluble | - |
Ovis aries | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.210 | 35000 | - |
gel filtration | Ovis aries |
| 1.1.1.210 | 36000 | - |
SDS-PAGE | Ovis aries |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.210 | Ovis aries | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.210 | - |
Ovis aries |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.210 | 5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ | 3beta and 20alpha substrates share the same active site | Ovis aries |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.210 | erythrocyte | fetal | Ovis aries | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.210 | 0.0096 | - |
20alpha-reductase activity | Ovis aries |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.210 | -20°C, purified enzyme, indefinitely stable | Ovis aries |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.210 | 17beta-hydoxy-5alpha-androstan-3-one + NADPH | - |
Ovis aries | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? |  |
| 1.1.1.210 | progesterone + NADPH | - |
Ovis aries | 20alpha-hydroxy-4-pregnen-3-one + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.210 | monomer | 1 * 36000, SDS-PAGE | Ovis aries |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.210 | NADP+ | - |
Ovis aries | |
| 1.1.1.210 | NADPH | - |
Ovis aries | |
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Release 2023.1 (January 2023)
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