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Literature summary extracted from

  • Chen, Q.; Nancarrow, C.D.; Sweet, F.
    Isolation of 3beta,20alpha-hydroxysteroid oxidoreductase from sheep fetal blood (1987), Steroids, 49, 477-496.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.210 stable to bromoacetic acid Ovis aries
1.1.1.210 stable to ethyl bromoacetate Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.210 16alpha-(Bromoacetoxy)progesterone competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation, 2-mercaptoethanol protects Ovis aries
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one inhibition of 20alpha-reductase activity Ovis aries
1.1.1.210 progesterone inhibition of 3beta-reductase activity Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.210 0.031
-
 progesterone
-
 Ovis aries
1.1.1.210 0.074
-
 17beta-hydroxy-5alpha-androstan-3-one
-
 Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.210 soluble
-
 Ovis aries
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.210 35000
-
 gel filtration Ovis aries
1.1.1.210 36000
-
 1 * 36000, SDS-PAGE Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one + NADPH Ovis aries
-
 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 progesterone + NADPH Ovis aries
-
 20alpha-hydroxy-4-pregnen-3-one + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.210 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.210
-
 Ovis aries

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.210 5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ 3beta and 20alpha substrates share the same active site Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.210 erythrocyte fetal Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.210 0.0071
-
 20alpha-reductase activity Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.210 17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
 Ovis aries 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
 Ovis aries 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 progesterone + NADPH
-
 Ovis aries 20alpha-hydroxy-4-pregnen-3-one + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.210 monomer 1 * 36000, SDS-PAGE Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.210 NADP+
-
 Ovis aries
1.1.1.210 NADPH
-
 Ovis aries