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Literature summary extracted from

  • Sharaf, M.A.; Sweet, F.
    Dual activity at an enzyme active site: 3beta,20alpha-hydroxysteroid oxidoreductase from fetal blood (1982), Biochemistry, 21, 4615-4620.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.210 lyophilization, complete loss of activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one inhibition of 20alpha-reductase activity Bos taurus
1.1.1.210 19-Nortestosterone 17-bromoacetate competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation Bos taurus
1.1.1.210 progesterone inhibition of 3beta-reductase activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.210 0.0025
-
 NADPH 20alpha-reductase Bos taurus
1.1.1.210 0.0094
-
 NADPH 3beta-reductase Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.210 soluble
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.210 55000
-
 SDS-PAGE, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one + NADPH Bos taurus
-
 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 progesterone + NADPH Bos taurus
-
 20alpha-hydroxy-4-pregnen-3-one + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.210 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.210
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.210 5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+ 3beta and 20alpha substrates share the same active site Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.210 erythrocyte fetal Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.210 -20°C, purified enzyme, indefinitely stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.210 17beta-hydoxy-5alpha-androstan-3-one + NADPH
-
 Bos taurus 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
 Bos taurus 5alpha-androstan-3beta,17beta-diol + NADP+
-
 ?
1.1.1.210 progesterone + NADPH
-
 Bos taurus 20alpha-hydroxy-4-pregnen-3-one + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.210 monomer 1 * 55000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.210 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.210 5.5 6
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.210 NADP+
-
 Bos taurus
1.1.1.210 NADPH
-
 Bos taurus