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Literature summary extracted from

  • Wagner, G.; Hollmann, S.
    Uronic acid dehydrogenase from Pseudomonas syringae. Purification and properties (1976), Eur. J. Biochem., 61, 589-596.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.203 DTT and dithioerythritol have protective function against thermal destruction Pseudomonas syringae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.203 KCN 15% inhibition at 1 mM Pseudomonas syringae
1.1.1.203 N-ethylmaleimide inhibition at 1 mM, reversal by an excess of L-cysteine Pseudomonas syringae
1.1.1.203 NADH competitive inhibition, Ki: 0.06 mM Pseudomonas syringae
1.1.1.203 p-chloromercuribenzoic acid 60% inhibition at 1 mM, reversal by an excess of L-cysteine Pseudomonas syringae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.203 0.054
-
 D-galacturonate
-
 Pseudomonas syringae
1.1.1.203 0.08
-
 NAD+
-
 Pseudomonas syringae
1.1.1.203 0.37
-
 D-glucuronate
-
 Pseudomonas syringae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.203 30500
-
 2 * 30500, SDS-PAGE, subunits alone inactive Pseudomonas syringae
1.1.1.203 59000
-
 gel filtration, ultracentrifugation Pseudomonas syringae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.203 D-glucuronate + NAD+ Pseudomonas syringae weak reversible reaction, 3% of the activity compared to the forward reaction D-glucaric acid 3,6-lactone + NADH + H+
-
 r
1.1.1.203 D-glucuronate + NAD+ + H2O Pseudomonas syringae
-
 D-glucaric acid + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Pseudomonas syringae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.203 to homogeneity, chromatography techniques Pseudomonas syringae

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.203 treatment with 8 M urea and 24 h dialysis: 10% of initial activity Pseudomonas syringae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.203 cell culture
-
 Pseudomonas syringae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.203 81
-
-
 Pseudomonas syringae

Storage Stability

EC Number Storage Stability Organism
1.1.1.203 -20°C, 50 mM sodium diphosphate-HCl, pH 8.0, 0.1% bovine serum albumin, no loss of activity Pseudomonas syringae
1.1.1.203 4°C, 50 mM sodium diphosphate-HCl, pH 8.0, 30% glycerol, 5% loss of activity within 1 month Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Pseudomonas syringae D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ weak reversible reaction, 3% of the activity compared to the forward reaction Pseudomonas syringae D-glucaric acid 3,6-lactone + NADH + H+
-
 r
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Pseudomonas syringae D-glucaric acid + NADH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.203 dimer 2 * 30500, SDS-PAGE, subunits alone inactive Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.203 37
-
-
 Pseudomonas syringae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.203
-
 15 stable for 24 h Pseudomonas syringae
1.1.1.203 25
-
 15% loss of activity within 2 h Pseudomonas syringae
1.1.1.203 37
-
 90% loss of activity within 60 min Pseudomonas syringae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.203 26.7
-
 D-galacturonate
-
 Pseudomonas syringae
1.1.1.203 80
-
 D-glucuronate
-
 Pseudomonas syringae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Pseudomonas syringae