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Literature summary extracted from

  • Tanimoto, T.; Ohta, M.; Tanaka, A.; Ikemoto, I.; Machida, T.
    Purification and characterization of human testis aldose and aldehyde reductase (1991), Int. J. Biochem., 23, 421-428.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 (NH4)2SO4 at 0.3 M more than 3fold activity Homo sapiens
1.1.1.21 (NH4)2SO4 0.1 M Homo sapiens
1.1.1.21 Na2SO4 at 0.3 M 3fold activity Homo sapiens
1.1.1.21 pyridoxal 5'-phosphate 2,5fold activity at 0,5 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 sodium valproate
-
 Homo sapiens
1.1.1.21 (E,E)-5[2-methyl-3-phenyl-2-propenylidene-4-oxo-2-thioxo-3-thiazolidine]acetic acid
-
 Homo sapiens
1.1.1.21 (S)-6-fluorospiro[chroman-4,5'-imidazolidine]-2',4'-dione
-
 Homo sapiens
1.1.1.21 N-[[6-methoxy-5(trifluoromethyl)-1-naphthalenyl]thioxomethyl]-N-methylglycine
-
 Homo sapiens
1.1.1.21 ponalrestat
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.0032
-
 NADPH
-
 Homo sapiens
1.1.1.21 0.025
-
 DL-glyceraldehyde
-
 Homo sapiens
1.1.1.21 0.25
-
 NADH
-
 Homo sapiens
1.1.1.21 5.05
-
 D-glucuronate
-
 Homo sapiens
1.1.1.21 6.2
-
 D-xylose
-
 Homo sapiens
1.1.1.21 49.3
-
 D-galactose
-
 Homo sapiens
1.1.1.21 57.3
-
 D-glucose
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 38000
-
 SDS-PAGE Homo sapiens
1.1.1.21 36000
-
 1 * 36000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 aldose + NAD(P)H Homo sapiens
-
 alditol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Homo sapiens
-
 human
-
1.1.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.2 testis
-
 Homo sapiens
-
1.1.1.21 testis
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 1.32
-
 purified enzyme, NADPH Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 additional information enzyme is virtually inactive for aldo-sugars Homo sapiens ?
-
 ?
1.1.1.21 alditol + NAD(P)+
-
 Homo sapiens aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Homo sapiens alditol + NAD(P)+
-
 r
1.1.1.21 aldose + NAD(P)H wide specificity Homo sapiens alditol + NAD(P)+
-
 r
1.1.1.21 D-galactose + NAD(P)H
-
 Homo sapiens dulcitol + NAD(P)+
-
 r
1.1.1.21 D-glucose + NAD(P)H
-
 Homo sapiens sorbitol + NAD(P)+
-
 r
1.1.1.21 D-glucuronate + NAD(P)H
-
 Homo sapiens ?
-
 r
1.1.1.21 D-xylose + NAD(P)H
-
 Homo sapiens xylitol + NAD(P)+
-
 r
1.1.1.21 DL-glyceraldehyde + NAD(P)H
-
 Homo sapiens glycerol + NAD(P)+
-
 r
1.1.1.21 xylitol + NAD(P)+
-
 Homo sapiens D-xylose + NAD(P)H + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 monomer
-
 Homo sapiens
1.1.1.21 monomer 1 * 36000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 0.183
-
 D-glucose
-
 Homo sapiens
1.1.1.21 0.233
-
 D-galactose
-
 Homo sapiens
1.1.1.21 0.431
-
 DL-glyceraldehyde
-
 Homo sapiens
1.1.1.21 1.7
-
 D-xylose
-
 Homo sapiens
1.1.1.21 2.3
-
 D-glucuronate
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADH
-
 Homo sapiens
1.1.1.21 NADPH
-
 Homo sapiens