EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.265 | KCl | 5-6fold increasing activity for NADPH-dependent activity at 0.2 M | Saccharomyces cerevisiae | |
1.1.1.265 | NaCl | 5-6fold increasing activity for NADPH-dependent activity at 0.2 M | Saccharomyces cerevisiae |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.265 | nutrition | essential in removal of the worthy off-flavours in beer during fermentation | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.265 | 2-methylbutanal | presence of NADPH, Ki: 2.74 mM, presence of NADH, Ki: 4.61 mM | Saccharomyces cerevisiae | |
1.1.1.265 | 3-Methylbutanal | presence of NADPH, Ki: 7.14 mM, presence of NADH, Ki: 253 mM | Saccharomyces cerevisiae | |
1.1.1.265 | Butanal | presence of NADPH, Ki: 16.6 mM | Saccharomyces cerevisiae | |
1.1.1.265 | Heptanal | presence of NADPH, Ki: 0.88 mM, presence of NADH, Ki: 1.17 mM | Saccharomyces cerevisiae | |
1.1.1.265 | hexanal | presence of NADPH, Ki: 0.79 mM, presence of NADH, Ki: 2.8 mM | Saccharomyces cerevisiae | |
1.1.1.265 | KCl | 5-6fold decreasing activity at 0.2 M for NADH-dependent activity | Saccharomyces cerevisiae | |
1.1.1.265 | NaCl | 5-6fold decreasing activity at 0.2 M for NADH-dependent activity | Saccharomyces cerevisiae | |
1.1.1.265 | NADP+ | complete inhibition at a 6.7fold excess of NADP+ in a reduction assay with 0.15 mM NADH | Saccharomyces cerevisiae | |
1.1.1.265 | pentanal | presence of NADPH, Ki: 3.9 mM, presence of NADH, Ki: 13.9 mM | Saccharomyces cerevisiae | |
1.1.1.265 | propanal | presence of NADPH, Ki: 46.6 mM | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.16 | - |
pentanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 0.18 | - |
hexanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 0.21 | - |
3-Methylbutanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 0.27 | - |
Heptanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 0.83 | - |
hexanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 1.22 | - |
Hexanol | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 1.69 | - |
trans-2-hexenol | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 1.85 | - |
2-methylbutanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 1.89 | - |
3-Methylbutanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 2.76 | - |
Butanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 3.01 | - |
pentanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 4.25 | - |
Heptanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 17.7 | - |
2-methylbutanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 23.1 | - |
Butanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 27 | - |
propanal | coenzyme NADH | Saccharomyces cerevisiae | |
1.1.1.2 | 38.9 | - |
propanal | coenzyme NADPH | Saccharomyces cerevisiae | |
1.1.1.2 | 158 | - |
acetaldehyde | - |
Saccharomyces cerevisiae | |
1.1.1.265 | 0.02 | - |
NADPH | - |
Saccharomyces cerevisiae | |
1.1.1.265 | 0.16 | - |
pentanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 0.18 | - |
hexanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 0.21 | - |
3-Methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 0.27 | - |
Heptanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 0.83 | - |
hexanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 1.22 | - |
Hexanol | presence of NADP+ | Saccharomyces cerevisiae | |
1.1.1.265 | 1.69 | - |
trans-2-hexenol | presence of NADP+ | Saccharomyces cerevisiae | |
1.1.1.265 | 1.7 | - |
NADH | - |
Saccharomyces cerevisiae | |
1.1.1.265 | 1.85 | - |
2-methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 1.89 | - |
3-Methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 2.76 | - |
Butanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 3.01 | - |
pentanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 4.25 | - |
Heptanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 17.7 | - |
2-methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 23.1 | - |
Butanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 27 | - |
propanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 38.9 | - |
propanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 158 | - |
acetaldehyde | presence of NADPH | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 37000 | - |
gel filtration | Saccharomyces cerevisiae |
1.1.1.265 | 37000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.265 | 3-methylbutanal + NAD(P)H | Saccharomyces cerevisiae | - |
3-methylbutanol + NAD(P)+ | - |
r | |
1.1.1.265 | acetaldehyde + NADPH + H+ | Saccharomyces cerevisiae | - |
ethanol + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Acinetobacter calcoaceticus | - |
- |
- |
1.1.1.2 | Saccharomyces cerevisiae | - |
yeast | - |
1.1.1.2 | Saccharomyces cerevisiae | - |
var. uvarum W34 | - |
1.1.1.265 | Saccharomyces cerevisiae | - |
brewer's yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | - |
Saccharomyces cerevisiae |
1.1.1.2 | partial | Acinetobacter calcoaceticus |
1.1.1.265 | to homogeneity, chromatography techniques | Saccharomyces cerevisiae |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.265 | cell culture | - |
Saccharomyces cerevisiae | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 61.6 | - |
- |
Saccharomyces cerevisiae |
1.1.1.265 | 61.6 | - |
in the presence of NADPH | Saccharomyces cerevisiae |
1.1.1.265 | 93.9 | - |
in the presence of NADH | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 2-methylbutanal + NADPH | - |
Saccharomyces cerevisiae | 2-methylbutanol + NADP+ | - |
r | |
1.1.1.2 | 3-methylbutanal + NADPH | - |
Saccharomyces cerevisiae | 3-methylbutanol + NADP+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
? | |
1.1.1.2 | hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae | hexanol + NADP+ | - |
r | |
1.1.1.2 | n-heptanol + NADP+ | - |
Saccharomyces cerevisiae | n-heptanal + NADPH | - |
r | |
1.1.1.2 | n-hexanol + NADP+ | - |
Saccharomyces cerevisiae | n-hexanal + NADPH | - |
r | |
1.1.1.2 | pentanal + NADPH | - |
Saccharomyces cerevisiae | pentanol + NADP+ | - |
r | |
1.1.1.2 | propanal + NADPH + H+ | - |
Saccharomyces cerevisiae | propanol + NADP+ | - |
r | |
1.1.1.2 | trans-2-hexenol + NADP+ | - |
Saccharomyces cerevisiae | trans-2-hexenal + NADPH | - |
r | |
1.1.1.265 | 2,3-pentanedione + NAD(P)H | very low activity | Saccharomyces cerevisiae | ? + NAD(P)+ | - |
? | |
1.1.1.265 | 2-methylbutanal + NAD(P)H | - |
Saccharomyces cerevisiae | 2-methylbutanol + NAD(P)+ | - |
r | |
1.1.1.265 | 3-methylbutanal + NAD(P)H | - |
Saccharomyces cerevisiae | 3-methylbutanol + NAD(P)+ | - |
r | |
1.1.1.265 | 3-methylbutanal + NADH + H+ | - |
Saccharomyces cerevisiae | 3-methylbutanol + NAD+ | - |
r | |
1.1.1.265 | acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ethanol + NADP+ | - |
r | |
1.1.1.265 | benzaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | benzyl alcohol + NAD(P)+ | - |
? | |
1.1.1.265 | butanal + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | butanol + NAD(P)+ | - |
r | |
1.1.1.265 | diacetyl + NAD(P)H | very low activity | Saccharomyces cerevisiae | ? + NAD(P)+ | - |
? | |
1.1.1.265 | furaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | furfuryl alcohol + NAD(P)+ | - |
? | |
1.1.1.265 | heptanal + NADPH | preference for long and branched-chain substrates with up to seven carbon atoms | Saccharomyces cerevisiae | heptanol + NADP+ | - |
r | |
1.1.1.265 | hexanal + NADPH + H+ | - |
Saccharomyces cerevisiae | hexanol + NADP+ | - |
r | |
1.1.1.265 | p-anisaldehyde + NAD(P)H | 20-50% of the activity with 3-methylbutanal for aromatic aldehydes | Saccharomyces cerevisiae | p-anisalcohol + NAD(P)+ | - |
? | |
1.1.1.265 | pentanal + NADPH | - |
Saccharomyces cerevisiae | pentanol + NADP+ | - |
r | |
1.1.1.265 | propanal + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | propanol + NAD(P)+ | - |
r | |
1.1.1.265 | trans-2-hexenol + NADP+ | - |
Saccharomyces cerevisiae | trans-2-hexenal + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | monomer | 1 * 37000, SDS-PAGE | Saccharomyces cerevisiae |
1.1.1.265 | monomer | 1 * 37000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.265 | bcADH | - |
Saccharomyces cerevisiae |
1.1.1.265 | branched-chain alcohol dehydrogenase | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.265 | 2.55 | - |
Hexanol | in the presence of NADP+ | Saccharomyces cerevisiae | |
1.1.1.265 | 5.6 | - |
acetaldehyde | in the presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 14 | - |
trans-2-hexenol | in the presence of NADP+ | Saccharomyces cerevisiae | |
1.1.1.265 | 47.3 | - |
Butanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 50.1 | - |
hexanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 55 | - |
propanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 56.6 | - |
pentanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 57 | - |
Butanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 71.1 | - |
hexanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 72.9 | - |
Heptanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 74.9 | - |
3-Methylbutanal | presence of NADPH | Saccharomyces cerevisiae | |
1.1.1.265 | 81.6 | - |
pentanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 84 | - |
propanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 91.8 | - |
3-Methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 92.3 | - |
2-methylbutanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 101 | - |
Heptanal | presence of NADH | Saccharomyces cerevisiae | |
1.1.1.265 | 113 | - |
2-methylbutanal | presence of NADPH | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.265 | 6 | 7 | NADH-dependent activity, highest activity at low ionic strength | Saccharomyces cerevisiae |
1.1.1.265 | 8.5 | - |
NADPH-dependent activity, highest activity at high ionic strength | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.265 | NADH | - |
Saccharomyces cerevisiae | |
1.1.1.265 | NADPH | preferred cofactor | Saccharomyces cerevisiae |