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Literature summary extracted from

  • Ma, K.; Robb, F.T.; Adams, M.W.
    Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis (1994), Appl. Environ. Microbiol., 60, 562-568.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.2 (NH4)2SO4
-
 Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 KCl
-
 Thermococcus litoralis
1.1.1.2 NaCl
-
 Thermococcus litoralis
1.1.1.2 potassium phosphate
-
 Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.033
-
 NADP+
-
 Thermococcus litoralis
1.1.1.2 0.1
-
 NADPH
-
 Thermococcus litoralis
1.1.1.2 0.4
-
 acetaldehyde
-
 Thermococcus litoralis
1.1.1.2 11.1
-
 ethanol
-
 Thermococcus litoralis
1.4.1.4 0.029
-
 NADP+ oxidative deamination at 80°C Thermococcus litoralis
1.4.1.4 0.14
-
 NADPH reductive amination at 80°C Thermococcus litoralis
1.4.1.4 0.16
-
 2-oxoglutarate reductive amination at 80°C Thermococcus litoralis
1.4.1.4 0.22
-
 L-glutamate oxidative deamination at 80°C Thermococcus litoralis
1.4.1.4 0.63
-
 NH4+ reductive amination at 80°C Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 48000
-
 4 * 48000, SDS-PAGE Thermococcus litoralis
1.1.1.2 175000
-
-
 Methanobacterium palustre
1.1.1.2 200000
-
 gel filtration Thermococcus litoralis
1.4.1.4 45000
-
 alpha6, 6 * 45000, SDS-PAGE Thermococcus litoralis
1.4.1.4 350000
-
 gel filtration Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Thermococcus litoralis
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Acetobacter aceti
-
-
-
1.1.1.2 Drosophila melanogaster
-
-
-
1.1.1.2 Methanobacterium palustre
-
-
-
1.1.1.2 Methanoculleus thermophilus
-
-
-
1.1.1.2 Methanofollis liminatans
-
-
-
1.1.1.2 Saccharomyces cerevisiae
-
 yeast
-
1.1.1.2 Thermoanaerobacter brockii
-
-
-
1.1.1.2 Thermococcus litoralis
-
 DSM 5473
-
1.1.1.2 Zymomonas mobilis
-
-
-
1.4.1.4 Thermococcus litoralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Acetobacter aceti
1.1.1.2
-
 Thermococcus litoralis
1.4.1.4
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 31.1
-
-
 Thermococcus litoralis
1.4.1.4 85
-
 oxidative deamination at 80°C and pH 8.0 Thermococcus litoralis

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 23°C, loses all of its activity within 1 week when stored anaerobically Thermococcus litoralis
1.1.1.2 4°C, about 1 week, 50% activity lost Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1,3-propanediol + NADP+
-
 Thermococcus litoralis ? + NADPH
-
 ?
1.1.1.2 1-octanol + NADP+
-
 Thermococcus litoralis octanal + NADPH
-
 r
1.1.1.2 1-pentanol + NADP+
-
 Thermococcus litoralis n-pentanal + NADPH
-
 r
1.1.1.2 ethanol + NADP+
-
 Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+
-
 Zymomonas mobilis acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+
-
 Thermococcus litoralis acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+
-
 Thermoanaerobacter brockii acetaldehyde + NADPH + H+
-
 r
1.1.1.2 isobutanol + NADP+
-
 Thermococcus litoralis isobutanal + NADPH + H+
-
 r
1.1.1.2 n-butanol + NADP+
-
 Zymomonas mobilis butyraldehyde + NADPH
-
 r
1.1.1.2 n-butanol + NADP+
-
 Thermococcus litoralis butyraldehyde + NADPH
-
 r
1.1.1.2 n-heptanol + NADP+
-
 Saccharomyces cerevisiae n-heptanal + NADPH
-
 r
1.1.1.2 n-heptanol + NADP+
-
 Thermococcus litoralis n-heptanal + NADPH
-
 r
1.1.1.2 n-hexanol + NADP+
-
 Saccharomyces cerevisiae n-hexanal + NADPH
-
 r
1.1.1.2 n-hexanol + NADP+
-
 Thermococcus litoralis n-hexanal + NADPH
-
 r
1.1.1.2 n-propanol + NADP+
-
 Thermococcus litoralis propionaldehyde + NADPH
-
 r
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Thermococcus litoralis L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Thermococcus litoralis 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer
-
 Saccharolobus solfataricus
1.1.1.2 tetramer
-
 Thermoanaerobacter brockii
1.1.1.2 tetramer
-
 Methanobacterium palustre
1.1.1.2 tetramer 4 * 48000, SDS-PAGE Thermococcus litoralis
1.4.1.4 homohexamer alpha6, 6 * 45000, SDS-PAGE Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 80
-
 ethanol oxidation Thermococcus litoralis
1.4.1.4 95
-
 oxidative deamination Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 85
-
 2 h, 50% activity lost Thermococcus litoralis
1.1.1.2 96
-
 15 min, 50% activity lost Thermococcus litoralis
1.1.1.2 98
-
 half-life 1-2 min Thermoanaerobacter brockii
1.4.1.4 85
-
 no activity loss after 5 h Thermococcus litoralis
1.4.1.4 98
-
 50% activity after 2 h Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 8.8
-
 ethanol oxidation Thermococcus litoralis
1.4.1.4 8
-
 oxidative deamination at 80°C Thermococcus litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NAD+
-
 Thermococcus litoralis
1.4.1.4 NADP+
-
 Thermococcus litoralis
1.4.1.4 NADPH
-
 Thermococcus litoralis