EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | synthesis | potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth | Thermoanaerobacter ethanolicus |
1.1.1.2 | synthesis | industrial ethanol production | Saccharomyces cerevisiae |
1.1.1.2 | synthesis | industrial ethanol production | Zymomonas mobilis |
1.1.1.2 | synthesis | industrial ethanol production | Thermoanaerobacter ethanolicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | 1° Adh cloned and characterized | Escherichia coli |
1.1.1.2 | 1° Adh cloned and characterized | Clostridium acetobutylicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.02 | - |
NADP+ | 1° Adh, ethanol as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.025 | - |
NADH | 1° Adh, acetaldehyde as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.033 | - |
NADPH | 2° Adh, propanone as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.049 | - |
NADP+ | 2° Adh, butan-2-ol as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.055 | - |
NADPH | 2° Adh, acetaldehyde as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.07 | - |
NADP+ | 2° Adh, ethanol as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.094 | - |
NADPH | 1°Adh, acetaldehyde as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.096 | - |
NAD+ | 1° Adh, ethanol as substrate | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.16 | - |
propanone | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.24 | - |
Butanone | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.25 | - |
acetaldehyde | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.44 | - |
Butanal | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.5 | - |
acetylCoA | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.51 | - |
butan-2-ol | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.56 | - |
propan-2-ol | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 1.6 | - |
formaldehyde | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 2.1 | - |
acetaldehyde | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 2.9 | - |
Butanal | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 10 | - |
butan-1-ol | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 19 | - |
n-Propanol | 2° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 23 | - |
n-Propanol | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 25 | - |
ethanol | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 34 | - |
n-butanol | 1° Adh | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 66 | - |
ethanol | 2° Adh | Thermoanaerobacter ethanolicus | |
1.2.1.10 | 0.029 | - |
n-butyryl-CoA | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 0.037 | - |
acetyl-CoA | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 0.063 | - |
NADH | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 0.16 | - |
CoA | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 0.44 | - |
NAD+ | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 1.3 | - |
acetaldehyde | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | 2.3 | - |
Butanal | - |
Thermoanaerobacter ethanolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 40000 | - |
4 * 40000, 2° Adh, secondary-alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | 42000 | - |
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | 96000 | - |
- |
Escherichia coli |
1.1.1.2 | 96000 | - |
- |
Clostridium acetobutylicum |
1.1.1.2 | 160000 | - |
2° Adh, gel filtration | Thermoanaerobacter ethanolicus |
1.1.1.2 | 170000 | - |
1° Adh, primary-alcohol dehydrogenase, gel filtration | Thermoanaerobacter ethanolicus |
1.2.1.10 | 100000 | - |
4 * 100000, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.2.1.10 | 360000 | - |
gel filtration | Thermoanaerobacter ethanolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.10 | acetaldehyde + CoA + NAD+ | Thermoanaerobacter ethanolicus | - |
acetyl-CoA + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Acetivibrio thermocellus | - |
- |
- |
1.1.1.2 | Clostridium acetobutylicum | - |
NRRL B643 | - |
1.1.1.2 | Clostridium acetobutylicum NRRL B643 | - |
NRRL B643 | - |
1.1.1.2 | Clostridium beijerinckii | - |
NRRL B592 | - |
1.1.1.2 | Clostridium beijerinckii NRRL B592 | - |
NRRL B592 | - |
1.1.1.2 | Clostridium kluyveri | - |
- |
- |
1.1.1.2 | Escherichia coli | - |
- |
- |
1.1.1.2 | Leuconostoc mesenteroides | - |
- |
- |
1.1.1.2 | Propionibacterium freudenreichii | - |
- |
- |
1.1.1.2 | Saccharomyces cerevisiae | - |
yeast | - |
1.1.1.2 | Thermoanaerobacter brockii | - |
- |
- |
1.1.1.2 | Thermoanaerobacter ethanolicus | - |
strain JW200 | - |
1.1.1.2 | Thermoanaerobacter ethanolicus | - |
39E(A.T.C.C. 33223), formerly Clostridium thermohydrosulfuricum 39E | - |
1.1.1.2 | Thermoanaerobacter ethanolicus JW 200 | - |
strain JW200 | - |
1.1.1.2 | Thermoanaerobacter thermohydrosulfuricus | - |
new name Thermoanaerobacter ethanolicus strain 39 E | - |
1.1.1.2 | Vibrio harveyi | - |
- |
- |
1.1.1.2 | Zymomonas mobilis | - |
- |
- |
1.2.1.10 | Thermoanaerobacter ethanolicus | - |
- |
- |
1.2.1.10 | Thermoanaerobacter ethanolicus 39E | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | 1° Adh and 2°Adh | Thermoanaerobacter ethanolicus |
1.2.1.10 | - |
Thermoanaerobacter ethanolicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 69 | - |
2° Adh | Thermoanaerobacter ethanolicus |
1.1.1.2 | 140 | - |
1° Adh | Thermoanaerobacter ethanolicus |
1.2.1.10 | 16 | - |
- |
Thermoanaerobacter ethanolicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.10 | 4°C, anaerobically | Thermoanaerobacter ethanolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 2-butanol + NADP+ | - |
Thermoanaerobacter brockii | butan-2-one + NADPH | - |
r | |
1.1.1.2 | 2-butanol + NADP+ | 2° Adh | Thermoanaerobacter ethanolicus | butan-2-one + NADPH | - |
r | |
1.1.1.2 | acetaldehyde + NADH + H+ | - |
Thermoanaerobacter ethanolicus | ethanol + NAD+ | - |
r | |
1.1.1.2 | acetaldehyde + NADH + H+ | - |
Thermoanaerobacter ethanolicus 39E | ethanol + NAD+ | - |
r | |
1.1.1.2 | acetaldehyde + NADH + H+ | - |
Thermoanaerobacter ethanolicus JW 200 | ethanol + NAD+ | - |
r | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Thermoanaerobacter ethanolicus 39E | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Thermoanaerobacter ethanolicus JW 200 | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetyl-CoA + NADPH | - |
Thermoanaerobacter ethanolicus | acetaldehyde + CoA + NADP+ | - |
? | |
1.1.1.2 | ethanol + NAD+ | - |
Thermoanaerobacter ethanolicus | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Escherichia coli | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Vibrio harveyi | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Leuconostoc mesenteroides | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Zymomonas mobilis | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter thermohydrosulfuricus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium kluyveri | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Propionibacterium freudenreichii | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium beijerinckii | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium acetobutylicum | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter ethanolicus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Acetivibrio thermocellus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter brockii | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium acetobutylicum NRRL B643 | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium beijerinckii NRRL B592 | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | formaldehyde + NADPH | - |
Thermoanaerobacter ethanolicus | methanol + NADP+ | - |
? | |
1.1.1.2 | formaldehyde + NADPH | - |
Thermoanaerobacter ethanolicus 39E | methanol + NADP+ | - |
? | |
1.1.1.2 | formaldehyde + NADPH | - |
Thermoanaerobacter ethanolicus JW 200 | methanol + NADP+ | - |
? | |
1.1.1.2 | additional information | 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA | Thermoanaerobacter ethanolicus | ? | - |
? | |
1.1.1.2 | additional information | 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA | Thermoanaerobacter ethanolicus 39E | ? | - |
? | |
1.1.1.2 | additional information | 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA | Thermoanaerobacter ethanolicus JW 200 | ? | - |
? | |
1.1.1.2 | n-butanol + NADP+ | - |
Zymomonas mobilis | butyraldehyde + NADPH | - |
r | |
1.1.1.2 | n-butanol + NADP+ | - |
Thermoanaerobacter ethanolicus | butyraldehyde + NADPH | - |
r | |
1.1.1.2 | n-butyraldehyde + NADPH | - |
Thermoanaerobacter ethanolicus | n-butanol + NADP+ | - |
r | |
1.1.1.2 | n-butyraldehyde + NADPH | - |
Thermoanaerobacter ethanolicus 39E | n-butanol + NADP+ | - |
r | |
1.1.1.2 | n-butyraldehyde + NADPH | - |
Thermoanaerobacter ethanolicus JW 200 | n-butanol + NADP+ | - |
r | |
1.1.1.2 | n-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | propionaldehyde + NADPH | - |
r | |
1.1.1.2 | propan-2-ol + NADP+ | 2° Adh | Thermoanaerobacter ethanolicus | propanone + NADPH | - |
r | |
1.2.1.10 | acetaldehyde + CoA + NAD+ | - |
Thermoanaerobacter ethanolicus | acetyl-CoA + NADH | - |
r | |
1.2.1.10 | n-butyraldehyde + CoA + NAD+ | - |
Thermoanaerobacter ethanolicus | n-butyryl-CoA + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | tetramer | - |
Propionibacterium freudenreichii |
1.1.1.2 | tetramer | - |
Thermoanaerobacter brockii |
1.1.1.2 | tetramer | 4 * 40000, 2° Adh, secondary-alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | tetramer | 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.2.1.10 | tetramer | 4 * 100000, SDS-PAGE | Thermoanaerobacter ethanolicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | 2° Adh | - |
Thermoanaerobacter ethanolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 6.5 | 9 | NAD(P)+ reduction | Thermoanaerobacter ethanolicus |
1.1.1.2 | 7 | - |
aldehyde-reduction | Thermoanaerobacter ethanolicus |
1.1.1.2 | 9 | - |
alcohol-oxidation | Thermoanaerobacter ethanolicus |
1.2.1.10 | 7 | - |
thioesterase activity | Thermoanaerobacter ethanolicus |
1.2.1.10 | 8.5 | - |
NAD+ reduction | Thermoanaerobacter ethanolicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.10 | CoA | - |
Thermoanaerobacter ethanolicus | |
1.2.1.10 | NAD+ | NADH-dependent | Thermoanaerobacter ethanolicus | |
1.2.1.10 | NADH | - |
Thermoanaerobacter ethanolicus |