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Literature summary extracted from

  • Yamada, H.; Shimizu, S.; Kataoka, M.; Sakai, H.; Miyoshi, T.
    A novel NADPH-dependent aldehyde reductase, catalyzing asymmetric reduction of beta-keto acid esters, from Sporobolomyces salmonicolor: purification and characterization (1990), FEMS Microbiol. Lett., 70, 45-48.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.2 synthesis useful for asymmetric production of L-carnitine Sporidiobolus salmonicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.2
-
 Sporidiobolus salmonicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.091
-
 ethyl 4-bromo-3-oxobutanoate
-
 Sporidiobolus salmonicolor
1.1.1.2 0.1
-
 octyl 4-chloro-3-oxobutanoate
-
 Sporidiobolus salmonicolor
1.1.1.2 0.36
-
 ethyl 4-chloro-3-oxobutanoate
-
 Sporidiobolus salmonicolor
1.1.1.2 1.3
-
 o-nitrobenzaldehyde
-
 Sporidiobolus salmonicolor
1.1.1.2 2.4
-
 3-Pyridinecarboxaldehyde
-
 Sporidiobolus salmonicolor
1.1.1.2 3
-
 methylglyoxal
-
 Sporidiobolus salmonicolor
1.1.1.2 4.4
-
 m-nitrobenzaldehyde
-
 Sporidiobolus salmonicolor
1.1.1.2 6.7
-
 p-chlorobenzaldehyde
-
 Sporidiobolus salmonicolor
1.1.1.2 7.3
-
 diacetyl
-
 Sporidiobolus salmonicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 32000
-
 gel filtration Sporidiobolus salmonicolor

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Sporidiobolus salmonicolor
-
-
-
1.1.1.2 Sporidiobolus salmonicolor AKU 4429
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Sporidiobolus salmonicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 81.7
-
-
 Sporidiobolus salmonicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 3-pyridinecarboxaldehyde + NADPH
-
 Sporidiobolus salmonicolor 3-pyridinemethanol + NADP+
-
 ir
1.1.1.2 D-glyceraldehyde + NADPH + H+
-
 Sporidiobolus salmonicolor glycerol + NADP+
-
 ir
1.1.1.2 D-glyceraldehyde + NADPH + H+
-
 Sporidiobolus salmonicolor AKU 4429 glycerol + NADP+
-
 ir
1.1.1.2 diacetyl + NADPH
-
 Sporidiobolus salmonicolor ? + NADP+
-
 ?
1.1.1.2 ethyl 4-bromo-3-oxobutanoate + NADPH
-
 Sporidiobolus salmonicolor (R)-4-bromo-3-hydroxybutanoic acid ethyl ester + NADP+
-
 ?
1.1.1.2 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Sporidiobolus salmonicolor (R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
 ?
1.1.1.2 m-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor m-nitrobenzylalcohol + NADP+
-
 ir
1.1.1.2 m-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor AKU 4429 m-nitrobenzylalcohol + NADP+
-
 ir
1.1.1.2 methylglyoxal + NADPH
-
 Sporidiobolus salmonicolor ? + NADP+
-
 ?
1.1.1.2 additional information D-glucuronate, D-glucose, D-xylose, D-galactose, formaldehyde, acetaldehyde, propionaldehyde, n-butyraldehyde, glycolaldehyde, acetone, acetol, lactaldehyde, dihydroacetone and acetoin are not reduced Sporidiobolus salmonicolor ?
-
 ?
1.1.1.2 additional information D-glucuronate, D-glucose, D-xylose, D-galactose, formaldehyde, acetaldehyde, propionaldehyde, n-butyraldehyde, glycolaldehyde, acetone, acetol, lactaldehyde, dihydroacetone and acetoin are not reduced Sporidiobolus salmonicolor AKU 4429 ?
-
 ?
1.1.1.2 o-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor o-nitrobenzylalcohol + NADP+
-
 ir
1.1.1.2 o-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor AKU 4429 o-nitrobenzylalcohol + NADP+
-
 ir
1.1.1.2 octyl 4-chloro-3-oxobutanoate + NADPH
-
 Sporidiobolus salmonicolor (R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
 ?
1.1.1.2 p-chlorobenzaldehyde + NADPH + H+
-
 Sporidiobolus salmonicolor p-chlorobenzyl alcohol + NADP+
-
 ir
1.1.1.2 p-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor p-nitrobenzylalcohol + NADP+
-
 ir
1.1.1.2 p-nitrobenzaldehyde + NADPH
-
 Sporidiobolus salmonicolor AKU 4429 p-nitrobenzylalcohol + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.2 monomer 1 * 32000, SDS-PAGE Sporidiobolus salmonicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 60
-
-
 Sporidiobolus salmonicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 55
-
 stable at pH 6.0-10.0 for 10 min Sporidiobolus salmonicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
-
 Sporidiobolus salmonicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.2 6 10
-
 Sporidiobolus salmonicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.2 6 10 stable at 55°C for 10 min Sporidiobolus salmonicolor
1.1.1.2 7
-
 stable below 60°C for 10 min Sporidiobolus salmonicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH highly specific for NADPH Sporidiobolus salmonicolor